当归藤原花青素的纯化工艺及体外抗氧化活性

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关键词：当归藤（EmbeliaparuifloraWall.）;原花青素；聚酰胺树脂；纯化工艺;抗氧化活性

中图分类号：R962 文献标识码：A

文章编号：0439-8114（2025）06-0155-06

DOI：10.14088/j.cnki.issn0439-8114.2025.06.026 开放科学（资源服务）标识码（OSID）：

Purification process and in vitro antioxidant activity of procyanidins from Embelia parviflora Wall.

FAN Li-hua 1 ，LIJin-zhou 2 ，LIMeng-qi'，ZHOUGuang-yun1，DENGQing-mei’，WEIJiang-cun³，CHEN Yongl

（1.GuangxiUniversity ofChineseMedicine，Nanning53O2Oo，China; 2.DrugResearchndeveotCnteofkanHspialAfliatedtagxiUivesityditioalCeseicin0， China;3.Guangxi International ZhuangMedical Hospital，Nanning53O2O1，China）

Abstract：ThepurificationprocessofproanthocyanidinsfromEmbeliaparuifloraWall.wasstudiedbyusing polyamideresinchromatography，andthestaticanddyamicadsorptionandesoptioncapacitisofolamidesinereinvestigated.TeantioxidantcapacityofthepurifedproanthocandinsfromEmbeliaparvifloraasalsoevaluated.Theresultsshowdthattheoptiizedpuificationpro cess wasasfolows：Thesampleloadingconcentrationwas1.88～3.21mg/L，thesampleloadingflowratewas2～3mL/min，thedynamic specific adsorption capacity of polyamide was 16.05mg/g ，and the optimal elution flow rate was 2～3 mL/min.The elution rates of different volume fractions of desorption solutions were： 50% ethanol solution >70% ethanol solution >30% ethanol solution >95% （204号 ethanolsolution.Intheantioxidant test，thescavengingratesofABS+·andthetotalreducingpowerofproanthocyanidinsfromEmbelidparifloraandtheproanthocyanidinreferencesubstancewereclosetothoseofvitaminC，butthescavengingratesofDPH·and superoxide anion were lower than those of vitamin C.

KeyWords：Embelia parviflora Wall；proanthocyanidins；polyamide resin；purification process；antioxidant activity

当归藤又名大力牛、虎尾草等，为紫金牛科（Myrsinaceae）酸藤子属（Embelia）小花酸藤子（Em-beliaparvifloraWall.）的干燥地上部分。（剩余9263字）