乙草胺和丁草胺对斑马鱼前列腺素代谢产物的影响

打开文本图片集

关键词：斑马鱼（Daniorerio）；前列腺素；代谢产物；乙草胺；丁草胺

中图分类号：X592；X174;Q959.4 文献标识码：A

文章编号：0439-8114（2025）06-0103-07

DOI：10.14088/j.cnki.issn0439-8114.2025.06.017 开放科学（资源服务）标识码（OSID）：

Effects of acetochlor and butachlor on prostaglandin metabolites in zebrafish

ZHOU Xin-zong，LI Shao-bin，TIAN Xiao-li，CHANG Ju-hua （CollegeofLifeScience，YangtzeUniversity，Jingzhou 434O25，Hubei，China）

Abstract：Adult female and male zebrafish（Danio rerio）were exposed to 100μg/L of acetochlor（AC）and butachlor（BC） for 96 h， andtheefectsofacetochlororbutachlorontheprostaglandin（PGs）metabolitesof zebrafishwereinvestigatedusing metabolomics techniques.Theresultsshowed thatan ultra-performance liquid chromatography-tandem massspectrometry（UPLC-MS/MS） detectionmethodwasestablshed，andtotalof1683metabolitesweredetectedfromthe24samplesofthesixtreatmentsACreated female（ACF）and malefish（ACM），BC treated female（BCF）and male fish（BCM），control female（CKF）and male fish（CKM）]. Volcano plotanalysisofallmetabolitesrevealedthatexceptforBCMandCKMtreatments，thenumberof significantlyup-regulated metaboliteswas notablyhigherthanthatofdown-regulatedones.Prostaglandinmetabolite（PGs）analysis showed thatunder ACF treatment，16PGsmetabolitesweresignificantlyup-regulated，andiPGsweresignificantlydown-regulated;underACMtreatent， 9PGs metabolites weresignificantlyup-regulated.AfterBCFtreatment，18PGs metabolites were significantlyup-regulated；after BCM treatment，2 PGs metabolites were significantly up-regulated.

Key Words：zebrafish（Danio rerio）;prostaglandins；metabolites；acetochlor；butachlor

前列腺素（PGs是一种类似激素的化学信使，参与人类和其他脊椎动物的多种生物过程，包括免疫系统调节，以及心血管、胃肠、泌尿生殖、内分泌、呼吸、性行为和生殖等过程[1，2]。（剩余9737字）