去亚甲基小檗碱对MPP+诱导的SH-SY5Y细胞线粒体功能损伤的保护作用

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[中图分类号] R338.2；R742.5 [文献标志码］A [文章编号]2096-5532（2025）03-0363-05doi：10.11712/jms.2096-5532.2025.61.098 [开放科学（资源服务）标识码（OSID）][网络出版] https：//link.cnki.net/urlid/37.1517.R.20250730.1149.008; 2025-07-3014：09：52

Protectiveefectofdemethyleneberberineagainstmitochondrialinjuryinducedby1-methyl-4-phenylpyridiniuminSH-SY5Yclls GONG Yongxin，LI Caiteng，ZHU Tianli，MA Zegang（Departmentof Physiology，Scholof Basic Medicine，Qingdao University，Qingdao 266071，China）

[Abstract]ObjetiveToinvestigate theprotective efectof demethyleneberberine（DMB）against mitochondrial injuryin duced by 1-methyl-4-phenylpyridinium （ MPP+ ）in SH-SY5Y cells.MethodsSH-SY5Y cells were divided into control group， （204号 MPP+ group，selegiline+MPP+ group，and DMB+MPP+ group. CCK8 assay was used to observe the effect of different concentrationsofDMBonthesurvivalrateofSH-SY5Ycels，andflowcytometrywasused to measure mitochondrialmembranepotential （2号 （Δψm） and reactive oxygen species （ROS）.ResultsThere was a reduction in the survival rate of SH-SY5Y cels after treatment with 1 mmol/L MPP+ for 24 hours，and different concentrations of DMB could improve the survival rate of SH-SY5Y cells， among which the co-treatment group with 10-3μmol/L DMB and MPP+ had the highest survival rate ⟨F=74.5，q=18.12，P< 0.001）. MPP+ treatment reduced Δψm in SH-SY5Y cells ⋅F=185.2，q=33.08，P<0.001） ，while both DMB and selegiline could reverse the effect of MPP+（q=12.97，15.32，P<0.001） 1 MPP+ treatment increased the content of ROS in cells （ ⋅F=79.2，q= ： ？0.88，P<0.001 ），while DMB and selegiline eliminated the effect of MPP+（q=14.62，15.08，P<0.001） .Conclusion DMB can alleviate mitochondrial injury in SH-SY5Y cells induced by MPP+ ：

[Keywords]berberine；Parkinson disease；mitochondria；selegiline

帕金森病（PD）作为一种神经退行性疾病，常伴有静止性震颤等症状，病理特征是中脑黑质致密部多巴胺（DA）能神经元的丢失等[1-2]。（剩余10384字）