A53T小鼠胰腺中GLP-1与 α-Syn 的表达变化

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[关键词] 帕金森病； αd 突触核蛋白；胰高血糖素样肽1：胰腺；小鼠，转基因[中图分类号］R338.2 [文献标志码]A [文章编号] 2096-5532（2025）03-0337-04doi：10.11712/jms.2096-5532.2025.61.083 [开放科学（资源服务）标识码（OSID）][网络出版]https：//link.cnki.net/urlid/37.1517.R.20250721.1427.001; 2025-07-21 16：35：23

Changes in the expression of glucagon-like peptide-1 and α -synuclein in the pancreas of A53T mice XU Haina，CHEN Xi （School of Basic Medicine，Qingdao University，Qingdao ，China）

[Abstract] Objective To investigate the changes in the expression of α⋅ synuclein （ α⋅ Syn） and glucagon-like peptide-1（GLP1）in the pancreas of A53T transgenic mice. MethodsA53T mice and wild-type（WT） mice，aged 6 months，were selected and their pancreatic tissue was collected. Western blotting was used to measure the protein expressionlevels of GLP-1 and α Syn in pancreatic tissue，and immunofluorescence assay was used to observe the localization of GLP-l and α Syn in pancreatic tissue. Re 1 sultsWesternblottingshowedthattherewas nosignificant diferenceintheexpressionlevelofGLP-1inpancreatictissuebetween A53T mice and WT mice （ P>0.05 . Immunofluorescence assay showed that GLP-1 and α -Syn were co-localized in pancreatic α cells，and compared with WT mice，A53T mice had a significantly higher expression level of α -Syn in pancreatic tissue （ Φt= 4.620，P<0.01） .ConclusionIn the pancreatic tissue of A53T mice， α Syn is co-expressed with GLP-1 in α -cells，and there is a significant increase in the expression level of α -Syn.

[Key words]Parkinson disease；alpha-synuclein； glucagon-like peptide-1；pancreas；mice，transgenic

帕金森病（PD）作为全球第二大神经退行性疾病，其典型病理特征为中枢黑质多巴胺能神经元丢失及 a- 突触核蛋白（ （α-Syn） 异常聚集[1-3]。（剩余8611字）