人参皂苷Rg1对TGF-β1诱导的星形胶质细胞反应性特性及细胞表型的影响
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[关键词］人参皂苷 Rg1 ；星形胶质细胞；转化生长因子β1；表型[中图分类号]R741;R284 [文献标志码]A [文章编号] 2096-5532（2025）03-0345-05doi：10.11712/jms.2096-5532.2025.61.097 [开放科学（资源服务）标识码（OSID）][网络出版] https：//link.cnki.net/urlid/37.1517.R.20250730.1031.003; 2025-07-3014：11：53
Effect of ginsenosideRg1onTGF-β1-induced reactivityand phenotypeof astrocytesHULirong，CHEN Wenfang，ZHENG Yangyang （Department of Physiologyand Pathophysiology，Schoolof Basic Medicine，Qingdao University，Qingdao 266071，China）
[Abstract]ObjectiveTo investigate the efectof ginsenosideRglonthereactivecharacteristicsand phenotypic transformationofastrocytes（As）inducedbytransforminggrowthfactor-beta1（TGF-β1）.MethodsAs wereisolatedfromthecerebral cortex of neonatal Sprague-Dawleyratsandwereinducedintoreactiveastrocytes（RAs）byTGF-βl，andthenRAsweretreated with ginsenoside Rg1 for 12 or 24 hours. The experiment was divided into control group，TGF-βl group，and TGF- group. Quantitative real-time PCR was used to measure the mRNA expresion levels of reactivity markers （Gfap，S10O），inflammatory factors（ IL Iβ ，TNF-a，iNOS），Al-typeAs markers （Gbp2，H2–T23） ，and A2-type As markers （SIOOAIO，Ptx3） ，andWesternbloting wasused to measure thechangesintheprotein expresionlevelsofGFAPandiNOS.ResultsGinsenosideRglsignificantly reduced the mRNA expression levels of Gfap ， SIOO ，iNOS， TNF–α ，and IL–Iβ （ F=11.09-136.70，q=6.43-16.01 ， P<0.05 ）and the protein expression levels of GFAP and iNOS in RAs （F=8.67，7.66，q=6.80，5.36，P<0.05） . Compared with the control group，the TGF- β1+Rg1 group had significant reductions in the A1-type markers Gbϕ2 and H2-T23（ F=61.94 ， 18.46，q=13.95，5.58，P<0.05） and significant increases in the A2-type markers S1ooA1o and Ptx3 ？ ′F=25.46，31.11，q=7.70 ， （204号 10.65，P<0.01） .Compared with the TGF- ⋅β1 group，the TGF group had significant reductions in the Al-type markers Gbp2 and H2 -T23 ⋅F=61.94，18.46，q=13.29，8.45，P<0.01） ，with no significant changes in A2-type markers（SlooA10， （20 Ptx3） .Conclusion Ginsenoside Rgl can inhibit TGF- ⋅β1 -induced reactive characteristics of As and promotes their phenotypic transformation from A1 phenotype to A2 phenotype.
[Key words]ginsenoside Rg1 ；astrocytes; transforming growth factor β1 ；phenotyp！
星形胶质细胞（As）作为中枢神经系统（CNS）中主要的神经胶质细胞，具有维持血脑屏障、突触形成与传递等作用[1-6]。（剩余10596字）
