6-羟基多巴胺对BV2小胶质细胞铁蛋白表达的影响

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[关键词] 羟多巴胺；铁蛋白质类；小神经胶质细胞；帕金森病[中图分类号]R742.5；R341.3 ［文献标志码］A [文章编号] 2096-5532（2025）03-0359-04doi：10.11712/jms.2096-5532.2025.61.078 [开放科学（资源服务）标识码（OSID）][网络出版]https：//link.cnki.net/urlid/37.1517.R.20250709.1559.005;

2025-07-10 11：55：34

Efect of 6-hydroxydopamineon the expressonofferitin inBV2 microglialcellsJING Cuiting，LIXiaoqian，QU Yan，XIE Junxia，WANG Jun（Departmentof Physiology，Scholof Basic Medicine，Qingdao University MedicalCollge，Qingdao 266071，China）

[Abstract]ObjetiveToinvestigate theeffctof6-hydroxydopamine（6-OHDA）ontheexpressionofferritin inBV2 microglialcell.MetodsAfterBV2microglialcelswere treated withdiffrentconentrationsof6-OHDA（1o，25，5Oμmol/L） for24hours，theamountoflactatedehydrogenase（DH）released was measured toreflectcellviability，andWesternblotwas usedtomeasurethechanges inthe protein expressionlevelsofferitin heavychain1（FTH1）andferrtinlightchain（FTL）in cells.ResultsCompared with thecontrol group without6-OHDA treatment，the BV2 microglial cels treated with 10μmol/L 6-OHDA for 24 hours had no significant change in the amount of LDH released，and the groups treated with 25 and 50μmol/L 6- OHDA and the maximum enzyme activity control group had a significant increase in the amount of LDH released ⋅F=92.750，q= （20号 6.799-24.780，P<0.001） . Compared with the control group，the BV2 microglial cells treated with 10μmol/L 6-OHDA for 24 hours had significant increases in the protein expression levels of FTHl and FTL （t=3.046，3.131，P<0.05） .ConclusionThis study shows that 6-OHDA can increase the expression of ferritin in BV2 microglial cells.

[Key words]oxidopamine；ferritins；microglia；Parkinson disease

帕金森病（PD）是第二常见的中枢神经系统退行性疾病[1]，黑质铁沉积是PD的重要病理表现[2]前期研究证实，在PD病人的中脑黑质有大量激活的小胶质细胞，这些细胞中存在大量铁沉积[3]。（剩余10429字）