MPTP和 α-Syn 预制原纤维诱导的慢性帕金森病模型小鼠黑质多巴胺能神经元的自发放电活动

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[中图分类号] R742.5；R338.8 [文献标志码］A [文章编号] 2096-5532（2025）03-0350-05doi：10.11712/jms.2096-5532.2025.61.082 [开放科学（资源服务）标识码（OSID）][网络出版]https：//link.cnki.net/urlid/37.1517.R.20250716.0942.001; 2025-07-1615：10：45

oontaneousdischargesofnigraldopaminergicneurons inmousemodelsofchronicParkinson’sdiseaseinducedbyMPTPand α -Syn

PFFsLIU Wenhong，LIU Cui，CHEN Xinyi， XUE Yan， CHEN Lei（Department of Physiology and Pathophysiology， 5chool of Basic Medicine，Qingdao Medical College of Qingdao University，Qingdao 266o71，China）

[Abstract]ObjectiveToinvestigate thespontaneous activityof substantianigra dopaminergic neurons in twomouse models of chronicParkinson’sdiseaseinducedby1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine（MPTP）andalpha-syucleinprefored fibrils （ α Syn PFFs），respectively.MethodsTwo mouse models of chronic Parkinson’s disease were prepared bylong-term injection of MPTP into the peritoneal cavity and stereotaxic injection of α -SynPFFs into the right striatum of the brain，respectively. Werecorded thespontaneousfiringactivityofdopaminergicneurons inthesubstantianigra parscompactaof the two modelsusing electrophysiology techniques. ResultsThe MPTP group and α Syn PFFs group showed significantly smaller mean numbers of firing dopaminergic neurons per recording session in the substantia nigra pars compacta （t=3.015，4.779 ，both P<0.05 ），significantly higher firing rates of nigral dopaminergic neurons ，both P<0.05 ），and significantly higher proportions of nigral dopaminergic neurons exhibiting burst firing （t=-3.611，-2.928 ，both P<0.05 ），compared to the control group. ConclusionMouse models of chronic Parkinson’s disease induced by MPTP and α Syn PFFs differ significantly from control mice， mainly manifesting withdecreasednumbersofneuronsexhibiting spontaneousfiringandincreasedfiringratesand proportionsof neurons exhibiting burst firing.

[Key words]substantianigra；dopaminergic neurons;alpha-synuclein；-methyl-4-phenyl-1，2，3，6-tetrahydropyridine;elec trophysiology；Parkinson disease；mice

黑质多巴胺能神经元的进行性缺失是帕金森病（PD）的特征性病理改变之一[1-2]。（剩余10824字）