ACSL4对MPP+处理后N2a细胞线粒体数量的影响

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[关键词]辅酶A连接酶类;1-甲基-4-苯基吡啶;线粒体;电压依赖性阴离子通道1;帕金森病[中图分类号］R338.2 [文献标志码］A [文章编号] 2096-5532（2025）03-0327-04doi：10.11712/jms.2096-5532.2025.61.081 [开放科学（资源服务）标识码（OSID）][网络出版]https：//link.cnki.net/urlid/37.1517.R.20250716.1027.004; 2025-07-1615：08：55

Efectof acy-CoAsynthetaselong-chainfamilymember4onmitochondrialnumberinN2acelsafter1-methyl-4-phenylpyridinim treatmentLIU Siqi，LIU Mengru，JIANG Hong（State KeyDisciplines：Physiology （Incubation），Departmentof Physiolo gy，Qingdao University，Qingdao ，China）

[Abstract]ObjectiveToinvestigate theefectofacyl-CoA synthetaselong-chain family member4（ACSL4）onmitochondrial number in N2a cells after1-methyl-4-phenylpyridinium （ .MPP+ ）treatment. MethodsWestern blotting was used to measure the protein expression level of ACSL4 in N2a cells after MPP+ treatment. N2a cels were transfected with Flag-Vector or FlagACSL4 plasmids，and Westernbloting wasused toobservethe efect of ACSL4overexpressonontheexpressonlevelsofvoltage dependentanionchanel1（VDAC1）and mitochondrial import receptor subunit TOM20 homolog（TOMM2o）in N2acels after （20号 MPP+ treatment. ResultsCompared with the control group，the MPP+ treatment group had a significant increase in the protein expression level of ACSL4 in N2a cells Ω′t=3.965，P<0.05） . Compared with the control group，the MPP+ treatment group had significant increases in the expresson levels of VDAC1 and TOMM20 （ F=28.59，15.15，P<0.01） ，while compared with the MPP+ treatment group，ACSL4 overexpression had no further impact on the expresson levels of VDAC1 and TOMM20 in N2a （204号 cells （P>0.05 ）.ConclusionACSL4 overexpression has no impact on mitochondrial number in N2a cells after MPP+ treatment，sugestingthattheroleof ACSL4inthepathogenesisofParkinson’sdiseasedoes not involveitsimpactonmitochondrial number.

[Key words] coenzyme A ligases;1-methyl-4-phenylpyridinium; mitochondria;voltage-dependent anion channel 1； Parkinson disease

帕金森病（PD）是一种常见的神经退行性疾病，主要病理特征为中脑黑质多巴胺能神经元选择性缺失，残存的神经元内出现以 α- 突触核蛋白（ ⋅a- Syn）异常聚集为主的路易小体[2]。（剩余8849字）