BV2小胶质细胞炎症对Dectin-1和iNOS表达影响

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[中图分类号］R742 [文献标志码]A [文章编号] 2096-5532（2025）03-0355-04doi：10.11712/jms.2096-5532.2025.61.079 [开放科学（资源服务）标识码（OSID）][网络出版] https：//link.cnki.net/urlid/37.1517.R.20250716.1026.003； 2025-07-1615：04：13

Efectof 1-methl-4-phenylpyidim-inducd iflammationontheexpresionofDecti-1andiducible nitricoxidesythaseiBV2 microglial cels ZHAO Ruiyue，WANG Xiaowen， YANG Zihao， ZHANG Rui，CHEN Wenfang， ZHANG Mei（Department of Physiology and Pathophysiology，School of Basic Medicine，Qingdao University，Qingdao 266071，China）

[Abstract] ObjectiveTo investigate the effect of 1-methy-4-phenylpyridinium （ MPP+ ）-induced inflammation on the expresionlevelsofDectin-1andinduciblenitricoxidesynthase（iNOS）inBV2microglialcels.MethodsBV2microglialcels were treated with different concentrations of MPP+（0.1，0.5 ，and 1.0mmol/L ，and MTT assay was used to measure cell viability. Western blotting was used to observe the effect of different durations of MPP+ treatment（2，4，6，12，and 24 hours）on the proteinexpressionlevelsofthepaternrecognitionreceptorDectin-1andtheinflammatoryfactoriNOSinBV2microglialcels. Results MPP+ at the concentration of 0.5mmol/L and 1.0mmol/L significantly reduced the viability of BV2 microglial cells （ （F=9.058;q=3.611，4.361;P<0.05） . There was a significant increase in the protein expression level of Dectin-1 at 4，6，and 12 hours after MPP+ induction （F=5.660，q=4.784-6.610，P<0.05） ，which returned to normal at 24 hours. There was a significant increase in the protein expression level of iNOS at 12 and 24 hours after MPP+ treatment （F=4.884;q=3.662，3.931;P< 0.05）．Conclusion MPP+ -induced inflammatory response in BV2 microglial cells can significantly increase the protein expression of the pattern recognition receptor Dectin-1 and iNOS.

[Key words]microglia；1-methyl-4-phenylpyridinium；receptors，patern recognition；nitricoxidesynthase typeI；neu roinflammatory diseases; neurodegenerative diseases

神经退行性疾病与神经炎症密切相关[]。（剩余8857字）