超声对PC12细胞的调控及对6-OHDA诱导的帕金森病小鼠模型运动行为的影响

打开文本图片集

[关键词」 帕金森病；超声波；PC12细胞；黑质；小鼠；运动活动[中图分类号]R338.2 [文献标志码］A [文章编号] 2096-5532（2025）03-0322-05doi：10.11712/jms.2096-5532.2025.61.076 [开放科学（资源服务）标识码（OSID）][网络出版]https：//link.cnki.net/urlid/37.1517.R.20250709.1559.003； 2025-07-10 14：18：01

Regulatory effects of ultrasoud onPC12cels and motorbhaviors of mouse modelof 6-OHDA-induced Parkinson’sdiseaseZHONG Guangjun，JIA Guorui，ZHAODi，XIEJunxia（Department of Physiologyand Pathophysiology，School of Basic Medicine， Qingdao Medical College of Qingdao University，Qingdao 266o71，China）

[Abstract]ObjectiveTo investigate the effects of ultrasound（US）on pheochromocytoma （PC12）celsand the motor behaviorsofamousemodelof6-hydroxydopamine（6-OHDA）inducedParkinson'sdisease.MethodsPC12celsweredividedinto control group and US group （stimulated with US），and the Ca2+ fluorescence intensity of the cells in the two groups was measured. C57BL/6J mice were divided into control group，model group，and US group to receive injection of normal saline，6-OHDA solution，and6-OHDA solutionintothestriatum，respectively；after2weeks，gaitanalysis wasperformedforthethre groups；and then，the US group was given US stimulation for 3d ，followed by gait analysis for the locomotion ability of the mice.Results PC12 cells in the US group showed a significant increase in the fluorescence intensity of Ca2+ than those in the control group （t= 3.071，P<0.05） . Compared with the model group，the mice in the US group showed a significantlylarger maximum contact area of the paws ⋅F=2.99，q=2.410，P<0.05） and a significantly higher movement frequency （F=40.35，q=3.879，P<0.01） . In theUS group，the step length of the mice was increased significantly after US stimulation compared with before US stimulation （ 2.636-9.760，P<0.05） . The content of tyrosine hydroxylase（TH） in the substantia nigra（SN） of the mice in the US group was significantly increased than that in the model group （F=78.03，q=3.589，P<0.05） ）but significantly lower than that in the control group .ConclusionUS can promote Ca2+ inflow in PCl2 cells，alleviate the movement disorder of PD mice，and save TH-positive neurons in the SN region.

[Key words]Parkinson disease；ultrasonic waves；PCl2 cells；substantia nigra；mice；motor activity

帕金森病（PD）是一种以黑质（SN）内多巴胺

（DA）能神经元进行性丢失为主要病理学特征的神经退行性疾病，其临床特征有运动迟缓、肌肉强直、静息性震颤、姿势跟步态障碍等[1]。（剩余10454字）