SNAP-94847经伏隔核对小鼠焦虑行为的影响

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[关键词]焦虑；行为症状；黑色素浓集激素1型受体；伏隔核；小鼠，近交C57BL[中图分类号]R749.72;R392.116[文献标志码]A[文章编号]2096-5532（2025）03-0331-06doi：10.11712/jms.2096-5532.2025.61.077 [开放科学（资源服务）标识码（OSID）][网络出版]https：//link.cnki.net/urlid/37.1517.R.20250709.1559.004； 2025-07-10 13：04：33

Efect of SNAP-94847 onanxiety-likebehaviorinmiceviathe nucleus accumbensFENG Xufei，WANG Xiaoxuan，TANGWen hui，WEILuling，GUOFeifei，LIUHua（DepartmentofPhysiologyandPathophysiology，SchoolofBasic Medicine，Qingdao University，Qingdao 266071，China）

[Abstract]ObjectiveTo investigate the efectof SNAP-94847，amelanin-concentrating hormone receptor blocker，onanxiety-like behaviorinmiceviathenucleusacumbens shell（NAcSh）．MethodsThechronic-acutecombining stress（CACS） method was usedtoestablishamouse modelofanxiety，andaccording tothe injectionof normal saline（NS）or SNAPinto the NAcSh，the CACS model mice and normal control（CON）mice were randomly divided into CON+NS group，CON+SNAP group，CACS + NS group，and CACS + SNAP group，with 8 mice in each group. The open field test （OFT），elevated plus maze （EPM），andthemarbleburyingtest（MBT）wereusedtoassessthechangesinrelatedindicesforanxiety-relatedbehaviorinmice after microinjectionof SNAP-94847intotheNAcSh.ResultsThere were significant diferencesinOFT，EPM，and MBTbetween all the groups （F=3.273-13.210，P<0.05） . Compared with the CON + NS group，the CACS + NS group had significant reductions in residence time and moving distance within the central region of OFT （ ⋅P<0.01） ，a significant reduction in total moving distance （ ΔP<0.05 ），significant reductions in the number of entries into the open arm and residence time in EPM（ P<0.01⟩ ： and a significant increase in the number of buried beads in MBT （ P<0.01 .Compared with the CACS + NS group，the CACS + SNAP grouphad significant increases inresidence time and moving distance inthecentralregion of OFT，significantincrease in thenumberofentriesintotheopenarmandresidencetimeinEPM，andasignificantreductioninthenumberofburiedbeads in MBT（all P<0.05 ）.ConclusionThere is a significant increase in anxiety-like behavior in CACS mice，and microinjection of SNAP-94847 into the NAcSh can improve such anxiety-like behavior.

[Key words] anxiety；behavioral symptoms；melanin concentrating hormone-l receptor；nucleus accumben；mice，inbred C57BL

焦虑是全球最常见的心理疾病之一，其特征是与实际威胁不成比例的持续性恐惧及担忧情绪[1-2]焦虑的形成与大脑边缘系统有密切的联系[3-4]。（剩余13753字）