大豆多肽发酵液脱色及不同超滤组分抗氧化研究

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摘 要：为探究豆渣发酵液的脱色效果和不同分子质量大豆多肽的体外抗氧化能力，利用枯草芽孢杆菌发酵豆渣酶解液制备大豆多肽，利用活性炭脱色处理，再采用超滤分离将豆渣多肽发酵液分级出

5～10 kDa，3～5 kDa，500～3 000 Da和

关键词：豆渣;大豆多肽;超滤;抗氧化活性

Study on Decolorization of Soybean Polypeptide Fermentation Broth and Antioxidant Activity of Different Ultrafiltration Components

LI Lele1， YANG Xuewei1， LUO Xueyun1， FAN Liu1， YIN Lebin1，2*

（1.School of Food and Chemical Engineering， Shaoyang University， Shaoyang 422000， China; 2.Hunan Provincial Key Laboratory of Soybean Products Processing and Safety Control， Shaoyang 422000， China）

Abstract： In order to explore the decolorization effect of soybean residue fermentation broth and the antioxidant capacity of soybean peptides with different molecular weight in vitro， soybean peptides were prepared from soybean residue enzymatic hydrolysate fermented by Bacillus subtilis， decolorized by activated carbon， and then separated by ultrafiltration. The soybean residue polypeptide fermentation broth was divided into 4 components of 5～10 kDa， 3～5 kDa， 500～3 000 Da and

5～10 kDa polypeptide component has the strongest scavenging activity against DPPH free radicals， and the

3～5 kDa polypeptide component has the strongest scavenging activity against -OH free radicals. This study provides theoretical guidance for the study of physiological activity of soybean peptides.

Keywords： bean dregs; soybean polypeptide; ultrafiltration; antioxidant activity

豆渣是豆制品加工副产物，其富含膳食纤维、有机酸和蛋白质等营养成分。（剩余5071字）