黑木耳黑色素提取工艺与来源对其UV-Vis光谱及抗氧化活性的影响

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中图分类号：S646.6 文献标志码：A 文章编号：1673-2871（2025）10-178-11

Effects of extraction process and source of Auricularia heimuer melanin on its UV-Vis spectrum and antioxidant activity

LI Yuling， CHEN Jiawen，LIU Kun'ang，MA Hong， ZHANG Genwei， LI Shusheng （InstituteofBiologyHebeiAcademyofSciences，ShijiazhuangO5oooo，HebeiChina）

Abstract：Auriculariaheimuerisatraditionaledibleand medicinal fungus，anditsmelaninexhibitsdiverse biological activities，includingantioxidant，antitumor，andimmunomodulatoryproperties.Topromotetheeficientdevelopentand utilization of A .heimuer resources，this study systematically evaluated melanin extracted from different sources and by variousmethodsbasedonyield，UV-Visspectralcharacteristics，andinvitroantioxidantactivity.Theresultsdemostrated thatenzymatichydrolysis Was theoptimal methodforextracting melanin fromfruitingbodies，achievingthehighestyield （ 9.49% ）and exhibiting the strongest antioxidant capacity.At a concentration of 2.0mg⋅mL⋅1 ，the melanin scavenged DPPH，ABTS， and O2- with efficienciesof 80.08% 82.87% ，and 63.47% ，respectively. In contrast，melanin derived from liquid fermentation broth exhibited superior scavenging activity against DPPH and O2-⋅ ，when the sample concentration was 2.0mg⋅mL-1 ，the scavenging rates were 98.52% and 80.61% ，respectively，and displayed UV-Vis spectral features mostsimilartothoseof the standard melaninreference.Thesefindings provideasolidtheoreticalfoundationforoptimizing the extraction ofA. heimuer melanin and advancing its application in functional product development.

KeyWords：Auricularia heimuer;Melanin;Extraction method;Melaninsource;Antioxidantactivity

黑木耳（Auriculariaheimuer）是一种传统的药食同源真菌，富含多糖、黑色素和多酚等生物活性成分，具有抗氧化、降血糖、保护心血管、抗肿瘤和提高免疫力等多种功能[1-]。（剩余15345字）