野生与栽培鸟巢蕨次生代谢组学差异分析

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中图分类号：S647 文献标志码：A 文章编号：1673-2871（2025）10-048-08

Abstract：To investigate thediferences insecondarymetabolitesofAspleniumnidus betweenwildandcultivatedmodes， a widely targeted metabolomicsanalysis was performed on leaf samplesofA.nidus from native（Group B）and cultivated （Group C）environments using ultra-high-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry（UHPLC-QTOF-MS/MS）.Differential metabolites were screened byorthogonal partial least squares discriminant analysis（OPLS-DA），followed bycorrelationanalysis and KEGG metabolic pathwayenrichment analysis.The results showed thatatotalof 647metabolites were identified inwildand cultivatedA.nidus，among which shikimicacid and phenylpropanoids accounted for the highest proportion （37% ），followed by terpenoids （ 19% ）andalkaloids（ 16% ）. Principal component analysis（PCA）and OPLS-DAresults indicated a significant separation in metabolic levels between the wild groupandthecultivatedgroup.Atotalof326diferentialmetaboliteswerescreened，including146up-regulated and180down-regulated，mainlyconcentratedinshikimalesand phenyloropanolds，alkaloids，andterpenoids.KEGG pathwayenrichment analysis revealed15significantly enriched metabolic pathways，among which flavonoidandflavonol biosynthesis，ABCtransporter pathways，and amino acid metabolism-related pathways showed the highest enrichment levels. Thefindings provideametabolomic basis foroptimizingthecultivationconditions ofA.nidus，improving thecontentof activecomponents，andevaluatingitsmedicinalvalue，ndalsolayafoundationforsubsequent explorationofitsmetabolic regulation mechanisms and development applications.

Keywords：Aspleniumnidus;Secondary metabolome;Orthogonalpartial least squares discriminantanalysis; Cultivated artificially

鸟巢蕨（Aspleniumnidus）作为铁角蕨科多年生蕨类植物，在我国南方地区既是传统药用资源，又因其嫩叶富含多糖、膳食纤维等成分被广泛食用[。（剩余10109字）