马铃薯A病毒适宜寄主的筛选与鉴定

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中图分类号：S532 文献标志码：A 文章编号：1673-2871（2025）10-140-08

Screening and identification of suitable host for potato virus A

ZHANG Wei， WEI Xuyan，FAN Guoquan， GAO Yanling， SUN Xuhong， BAI Yanju （HeilongjiangAcademyofAgriculturalciences，Harbin5oo86，Heilongjiang，China）

Abstract： Potato virus A（PVA）is one of the significant viruses infecting potato，belonging to the Potyvirus genus，and can reduce global potato yields by 30%-40% . Understanding the identification，propagation hosts，and biological characteristicsofPVAiscrucial forstudying itspathogenic mechanismsand developing efectivecontrol strategies.Inthis study，PVA wasinoculated into18 indicator plantsusing thefriction inoculationmethod.Throughcombinedobservation ofbiological symptomsandRT-PCRdetection，systemically infected propagation hosts and locally infecteddiagnostic hosts were screened.TaqManreal-timequantitativePCR（TaqManRT-qPCR）wasemployed to track thediseaseprogression inpropagation hosts，studying thechanges inviralcontentand biological manifestationsofvirusaccumulationate in differenthosts.Byintegratinghostsize，totalleafarea，andviralaccumulationdynamics，theoptimalpropagationhostfor PVA was identified.The results showed that Physalis floridana developed localized necrotic spots on inoculated leaves， makingitatabledagnosticostfor.icondraysaloides，icotinanaebneyi，icotianrusticacot tabacum，and Nicotiana tasacumca sanctumexhibited systemic infectionsand wereidentifiedas propagation hosts.Consideringplantsize，total leafarea，andviralaccumulationrates，Nicotianarustica emergedasthe mostsuitablepropagation host for PVA.This study provides basic datas for further research and scientific prevention on PVA.

Keywords：PotatovirusA;TaqManRT-qPCR;Identifyhost;Breedinghost

马铃薯（SolanumtuberosumL.）是全球及中国第四大粮食作物，仅次于水稻、小麦和玉米。（剩余12515字）