香菇菌糠木质纤维素酶解产糖工艺优化及组成分析

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中图分类号： S646.1+2 文献标志码：A 文章编号：1673-2871（2025）10-068-07

Abstract：InordertoenhancetheutilizationeficiencyofspentLentinus edodes substrate，this studyused itasthemain raw material to produce sugars through lignocellose enzymatic hydrolysis.Key process conditions were optimized using single-factorandortogonaldsignexperiments，andcangesinlignocelluloseompositionaswellasinfraredctro copycharacteristicsbefore andafter enzymatic hydrolysis werecompared.Theresults showed that theoptimalconditions for sugar production from lignocelulose in spent Lentinus edodes substrate were as follws： Compound enzyme（celulase/ xylanase ）ratio of1：1，compound enzyme addition of 3000U⋅g-1 ，solid-liquid ratio of1：2，and enzymolysis timeof 8h ： Under these conditions，the reducing sugar content reached 5.472% ，whichwas2.304timeshigherthanthatoftheraw materials.After enzymatic hydrolysis，the content of hemicellulose and celllose decreased by 26.564% and 12.105% respectively.Infraredspectroscopyanalyaisrevealedthatthecharacteristicabsorptionpeaksofthelignocelllosefunctional groups remainedunchangeed inposition，but weakened inintensityafter enzymatic hydrolysis， which was consistent with thedegradation trendsofthetwocomponent.Inconclusion，enzymatichydrolysiseectivelyincreasedtheyieldofeducing sugar and promoted the degradation of lignocellulose in spent L .edodes substrate.This studyprovides a scientific basis for the high-value utilization of spent L .edodessubstrate.

Key words： Spent L. edodes substrate; Enzymatic hydrolysis; Lignocellulose; Infrared spectroscopy

香菇是我国重要的常规主栽食用菌种类，也是我国产量最高的食用菌。（剩余10104字）