不同方式长期保藏黑木耳菌种性状的效果

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中图分类号：S646.6 文献标志码：A 文章编号：1673-2871（2025）10-171-07

Abstract：Tostudytheeffctsofdifferent preservation methodsonthe traitsofAuricularia heimuerstrains，1Ostraisof A.heimuer that had been preserved for2O years by liquid nitrogen preservation method and 4∘C slant transplantation methodrespectively were used as thetest materials.Methods such as determinationof growth status，cultivationfor fruiting bodyproduction，esterase isoenzyme analysis，and ISSR molecularmarkeranalysis wereadopted to investigate the long-term preservationeffectsofdiferent preservation methods.Theresults showed thatfor thesame strain，in termsof mycelialgrowthvigorandgrowthrate，theoverallperformanceof liquid nitrogen preservation was significantlybetter than that of the 4∘C slant transplantation method.There was no significant diffrence in the aspect of fruiting body production.For thesame strainunder different preservation methods，theclusteringanalysisof the esterase isoenzyme indicated wasuniformlyl，andthesimilaritycoefficients inthe ISSRmolecular markerclusteringanalysis Wereall higher thanO.90.Thisindicates that thesamestrainunderdiferentpreservationmethodsishighlysimilarbuttherearestillcertain diferences.It is likely that changes occurred during the long-term preservation of the strain by the 4∘C slant transplantationmethodor during thesubculture process.This studyisof great significanceforthe long-term preservationof ΠM heimuer strains and maintaining stability during the process of industrial production.

KeyWords：Auricularia heimuer;Strain preservation;ISSR marker;Esterase isoenzyme

黑木耳（Auriculariaheimuer）是我国第二大食用菌生产菌种，味道鲜美，营养丰富[1]，营养价值和药用价值极高2，具有抗肿瘤、抗氧化、降血糖、降血脂等功效[3-5]。（剩余7639字）