多黏类芽孢杆菌YF对番茄灰霉病的生防机制初探

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中图分类号：S641.2 文献标志码：A 文章编号：1673-2871（2025）10-061-07

Preliminary study on the biocontrol mechanism of Paenibacillus polymyxa YF against tomato gray mold

ZHANG Jianqiang， WEI Tao，LAI Xin， WANG Lihua， LIANG Jian， TANG Gon{ （CollegeofResourcesndEnvironment，AbaTeachersCollege，Aba642，ichuanCina）

Abstract：TomatograymoldisadevastatingfungaldiseasecausedbyBotrytiscinerea，whichisoneoftheimportantfactors restricting tomatoyieldandquality.This study focusedonBotrytis cinereaand its biocontrolbacteria wereusedas theresearchobject，andexplored thecontroleffectandmechanismof thebiocontrolbacteria.Plateconfrontationassays showed that Paenibacillus polymyxa YF exhibited an inhibition rate of 41.04% against B .cinerea.Microscope observation revealedsignificantmorphologicalabnormalitiesinthehyphaeatthecononyedges，characterizedbythickenedhyphae，increased branching，shortened internoded，andthe presenceofvesicle-like structures.Enzymeactivityplatedetection further showed that P. polymyxa YF could produce chitinase， β -1，3-glucanase and protease.When the fermentation broth was extracted with n-butanol and the extract was dissolved in methanol to 1mg⋅mL-1 solution for plant confrontation assyas， the inhibition rate increased to 67.86% . Inaddtion，strain YF exhibited broad-spectrum inhibitory activity against eight common plant pathogens，with the lowest inhibition rate was 57.69% against Sclerotinia sclerotiorum and the highest of （204号 68.57% against Rhizoctonia solani. In summary， P. polymyxa YF has significant antagonistic activity and antibacterial substancesecretionability，providingahighlypromisingbiocontrolstrainforthebiologicalcontrolof tomato graymold.

Keywords：Tomato;Botrytiscinerea;Biological control; Paenibacillus polymyxa; Control mechanism

番茄是重要的蔬菜作物，全球年总产量达1.7亿t，在蔬菜作物中位居首位。（剩余13112字）