干扰转录因子StMYB90-like对彩色马铃薯花青素合成的影响

打开文本图片集

中图分类号：S532 文献标志码：A 文章编号：1673-2871（2025）10-056-05

Abstract：Toclarifytheregulatorymechanismof thetranscription factor StMYB9O-like in anthocyanin synthesis and metabolism，thisstudyusedthecoloredpotatoHeijingangasthematerial.Basedonthepreviouslyidentified StMYB90-like gene through bioinformaticsscreening，aninterference vector wasfurtherconstructedand geneticalytransformed.Through fluorescencequantitativeanalysis，anthocyanin content determination，and performanceobservationof transgenic plants，it was foundthat interference with StMYB90-likeexpresion significantlyreduced their transcription levels.Atthe same time，theanthocyanincontentinleavesdecreased by 47.94% ，thecolorof tubersbecame lighter，and the anthocyanin content in tubers decreased by 9.79% .Theaboveresultsindicate that the gene StMYB9o-like positively regulatesanthocyanin synthesis incolored potatoes，likelybyupregulating the expressonofstructural genes involved in anthocyanin biosynthesis，thereby modulating the synthesis and accumulation of anthocyanins in potato tissues.

Keywords： Colored potato; Anthocyanin; Genetic transformation; Transcription factor; StMYB90-like

马铃薯种质资源非常丰富。（剩余7788字）