不同施肥方式对党参土壤微生物多样性的影响研究

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中图分类号：R282 文献标志码：A 文章编号：2096-9902（2025）16-0031-06

Abstract：TakingCodonopsispilosulaplantingsoilwithcontinuouscroppingobstaclesastheresearchobject，fourtreatments were setup： blank control（CK），single application of compound fertilizer（DS1），compound fertilizer + microbial inoculant 1 （DS2），and compound fertilizer + microbial inoculant 2（DS3）. Iumina NovaSeq 6OOO sequencing technology was used' to analyze theefectsofdiferentfertlizationmethodsonthemicrobialcommunitystructureanddiversityofCodonopsispilosulasoil.The resultsshowedthattheombined treatmentofcompoundfertilizersandmicrobialinoculants（S2，DS3）significantlyimprovedthe speciesrichnessanddiversityofthebacterialcommunity，andpromotedfunctionalgroupsrelatedtocarbonandnitrogencycles suchasAcidobacteriotaandVerrucomicrobiotaaswellastheenichmentofnitrogen-fixingandphosphorus-disolvingbacteria suchasBlastomonasandcandidateUdaea，reshaping thesoil bacterialcommunitystructure.Singleaplicationofcompound fertlizer（DS）promotedtheexpansionoffungalcommunitiesitheshortterm，anditsspeciesichnessassignificantlyhigher thanothertreatments，butthecombinedtreatmenthadlitleimpactonfungaldiversityAttheFungallevel，therelative abundaneofAscomycotaincreasedinDS1andDS3，andtheBasidiomycetesdecreasedsignificantly；atthegenuslevel， beneficialgroupssuchasMortierellwereenrichedinthecombinedtreatment，buttheabundanceofpathogenssuchasFusarium hasalsoincreasedequringatentiontolong-termecologicalrisks.Betadiversityanalysisshowedthatthecombinedtreatment increasedthedispersionofbacterialcommunitiesbyintroducingexogenousfunctionalbacteria，whilesingleaplicationof compound fertilizer may inhibit some indigenous bacteria.

Keywords：Codonopsispilosula;compoundfertlizer;microbialinoculant；continuouscroppingobstacle；soilmicrobialdivesity

党参（Codonopsispilosula（Franch.）Nannf.）是我国传统中药材，具有健脾益肺、养血生津的作用，在中医药领域占据着重要地位。（剩余8272字）