分子生物学技术在食品微生物检测中的应用

摘 要：本文详细探讨了聚合酶链反应、实时荧光定量聚合酶链式反应、扩增片段长度多态性分析、核酸适配体以及CRISPR-Cas系统等分子生物学技术在食品微生物检测中的应用，总结出这些技术的具有检测敏感度高、特异性好、检测时间短，以及能够扩大检测范围等优势，可为食品安全检测提供高效、灵活的工具。

关键词：分子生物学；食品；微生物检测

Application of Molecular Biology Techniques in Food Microbial Detection

JIAO Xianjun， DONG Guihua

（Department of Microbiology， Zhangqiu Center for Disease Control and Prevention， Jinan 250200， China）

Abstract： In this paper， the application of molecular biology techniques such as polymerase chain reaction， real-time polymerase chain reaction， amplified fragment length polymorphism analysis， aptamers and CRISPR-Cas systems in the detection of food microorganisms is discussed in detail， and it is concluded that these technologies have the advantages of high detection sensitivity， good specificity， short detection time， and can expand the detection range， which can provide efficient and flexible tools for food safety detection.

Keywords： molecular biology; food; microbial detection

近年来，食品安全问题日益突出，已受到消费者的广泛关注。（剩余4551字）