荞麦种间杂交群体基因定位解析
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关键词：荞麦（FagopyrumesculentumMoench.）;种间杂交群体;基因组测序;基因定位中图分类号：S334 文献标识码：A文章编号：0439-8114（2025）05-0167-06DOI：10.14088/j.cnki.issn0439-8114.2025.05.026
Genetic mapping analysis of interspecific hybrid population in buckwheat
{INGui-fang1，LUWen-jiel，LONG Wen-jie'，SUNDao-wang1，HUANGChun-yan²，ZHAIBing-xin2，WANGLi-ua’ （1.BiotechnologndGeneticGemplasmResourcesResearchIsituteYunanProvincialKeyLaboratoryofgiculturaloteologKey LaboratoryofSouthwesteCropGeneResouresandGermplasmInnovation，MinistryofAgricultureandRuralAfairs，YunnanAcademyof AgriculturalSciences，Kunming 650205，China;2.Instituteof Resource Plants，Yunnan University，Kunming6505oo，China）
Abstract ： An F2 population was constructed by crossng the cultivated buckwheat（Fagopyrum esculentum Moench.）variety Yunqiao 1 （YQ1）withthelodging-resistantanddisease-resistantwildbuckwheatYZ56，extremephenotypicindividualsfortraitsincludingplantheight，rainweightperplant，ndleafblightresistancewerescreenedbasedonagronomictraitinvestigationdataadDA wasextractedfrombuckwheatleavesusing theCTABmethodtoconstruct extremephenotypicDNApools.Theresultsdemonstrated that plant height and grain weight per plant in the F2 population showed normal distributions，with individuals exhibiting lowerleaf blightincidenceoccupyingahigherproportioninthedistribution.Theaverage grainweightperplantoftheF2populationwas7.8g， higherthnthatof heparentalinesYZ56（13.1g）andYQ1（16.7g），indicatingsignificantheterosis.Rationalutilizationofdistant germplasmesourcesforhybridizationcouldimproveprogenyyield.Pearsoncorelationaalysisrevealedhighlysignificantcoelations amongplantheight，grainweightperplant，andleafblightincidencerate.Poledsequencingcombinedwithbulkedsegregantanalysis （BSA）successfully identified 1O QTL regions associated with plant height at a 95% confidence interval，7 candidate QTL regions related to grain weight per plant，and 6 candidate QTL regions linked to leaf blight resistance.
Key words：buckwheat（Fagopyrum esculentum Moench.）；interspecific hybrid population;genome sequencing;genemapping
荞麦（FagopyrumesculentumMoench.）是蓼科荞麦属1年生或多年生草本或半灌木植物，其中苦荞和甜荞是2种广泛栽培的重要亚种[1-3]。（剩余6127字）