早孕因子单克隆抗体对宫颈癌HeLa细胞增殖、迁移及调亡的影响

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关键词：早孕因子；单克隆抗体；宫颈癌；HeLa细胞；增殖；迁移；凋亡中图分类号：R711.74 文献标识码：A文章编号：0439-8114（2025）05-0117-06DOI：10.14088/j.cnki.issn0439-8114.2025.05.018

Effects of monoclonal antibody against early pregnancy factor on proliferation，migration and apoptosisofcervical cancer HeLa cells

DUANFu-chun，REN Hong-lin，HU Pan，LI Yan-song，LIUXi-lin，WANGHan， LIU Meng-di，LI Hao-song，LU Shi-ying

eyLaboratoryforDiagnosisandTreatmentofSevereZoonoticInfectiousDiseases/KeyLabratoryofZoonosisResearch，Ministryof Education/Instituteof Zoonosis/College of VeterinaryMedicine，JilinUniversity，Changchun13oo62，China）

Abstract：Theinibitoryefectofmonoclonalantibodyagainstearlypregnancyfactor（EPF-B6）onthebiologicalbehaviorofcervical cancer HeLacellswasinvestigatedbystudyingtheeectsofEPF-B6ontheproliferation，migration，cloningandapoptosisofHeLa cells.RT-PCR and Western Blot were used to detect the expression of EPF gene in six common cancer cells and EPF protein in cell culture medium.TheeffctsofdiferentconcenrationsofEPF-B6monolonalantibodyontheproliferation，migrationndpopulation dependenceofHeLacelsweredetectedbyCCK8assaycellscrathassayandplatecloning assay，espectively.Theapoptosisand cycleofHeLacellswere detected by flowcytometry.Results showed that EPF gene was present in common tumor cells.EPF protein was expresed in theculture medium ofcervicalcancer HeLacells，breastcancer MCF-7celsandovariancancer A2780cels.The resultsshowedthatEPF-B6monoclonalantibodycouldsignificantlyinhibit theproliferation，migrationandcolonyformationofHeLa cells，it was concentration-dependent （ P<0.01 ）.The results of cell cycle analysis showed that EPF-B6 monoclonal antibody blocked the DNA replication process of HeLa cells at G2/M phase.

Key Words：earlypregnancyfactors；monoclonalantibody;cervicalcancer;Helacells；proliferation;migration；apoptosis

早孕因子（Earlypregnancy factor，EPF）是澳大利亚学者在孕鼠血清中首次发现的，是一种具有免疫抑制和生长调节作用的微量蛋白[12]，其与肿瘤的发生密切相关。（剩余9682字）