受旱对水稻和土壤镉含量及镉抗性相关微生物的影响

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关键词：镉；受旱；水稻（OryzasativaL.）；土壤；镉抗性微生物；微生物多样性

中图分类号：S511；X53；S154.3 文献标识码：A

文章编号：0439-8114（2025）05-0010-07

DOI：10.14088/j.cnki.issn0439-8114.2025.05.003 开放科学（资源服务）标识码（OSID）：

Effects of drought on cadmium content in rice and soiland cadmium resistance-associated microorganisms

ZHANG Hui，WANG Yi，HANG Xiao-ning，ZHANG Jian，LIAO Dun-xiu，TANG Rong-li AcademyofAgriculturalSciences，hongqing401329，Chin

Abstract：Sampleswerecolectedfromtypicaldought-affectedfieldsinmid-altituderegions，ncludingrice（OryasatiaL.）plants andsoilsundermild，moderateandseveredroughtstressThecontentofcadmiuminsoilanddiffrentpartsofricewasdeteied， andthecommunitydiversityandcompositionofsoilcadmium-resistant microrganisms wereanalyzedbyIlumina MiSeq technique. Theresultsshowedthatecadmiumcontentinsoilwasintheincreasingorderofseveredrought，moderatedroughtandlightdrought， andthecadmiumcontentinachpartofriceshowedtheoppositetrend，butthediferenceswereallnotsignficant.Thesultsof high-throughputsequencingshowedthatthenumberofcadmiumresistance-asociatedmicroorganismswasthehighestundermoderatedrought，followedbymilddroughtandtheleastunderseveredrought.NMDSanalysisshowedthatdrought exposuresignificantly affected thestructureofcadmiumresistance-associatedmicrobialcommunities.Theanalysisofmicrobialcommunitiesshowedthat Proteobacteria，PlanctomycetesandActiobacteriawerethemainmicrobialphylainthethregroups，buttherelativeabundanceof eachphylawasdiferent.Underseveredrought，therelativeabundanceofPlanctomyceteswassignificantlyincreased，whilethelativeabundanceofProteobacteriadecreased.Atthegenuslevel，Gemmatawasthemostdominantmicrorganismacrossallhree drought-treatedgroups.Notably，itsabundanceinseveredroughtsoilwassignificantlyhigherthanthatinmildandmoderatedrought soils.Undermoderatedrought，theelativeabundaneofAzospirillmincreasedsignificantly，whereasundermilddought，theelativeabundancsofHaliangium，seudomonas，ndAloactinosynemasowedsignificantincreases.SeveredroughtincreasedtheCd contentofbrownriceandafectedthestructureandcompositionofcadmiumresistanceassociated microbialcommunities.Therefore， properwater management was expected toreduce the migrationofheavy metals insoilandrealizethesafeuseofcultivatedland.

Key Words：cadmium；drought;rice（Oryza sativaL.）；soil;Cadmium-resistant microorganism；microbial diversity

土壤镉（Cd）污染严重威胁中国稻田安全生产。（剩余12762字）