耐硒菌株BacilluscereusZJ2的筛选及四种碳源对其合成纳米硒稳定性的影响

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关键词：耐硒菌株；蜡样芽孢杆菌（Bacilluscereus）；纳米硒；还原率；稳定性；碳源中图分类号：S154.3 文献标识码：A文章编号：0439-8114（2025）05-0025-08DOI：10.14088/j.cnki.issn0439-8114.2025.05.00：

Isolation of selenium-resistant strain Bacillus cereus ZJ2 and the effects of four carbon sources on the stability of selenium nanoparticles synthesized by the strain

HE Jia-li，LIU Meng-qi， ZHONG Wen-yi，HE Yi，WANG Zhang-qian，CHENG Shui-yuan （ScholofModeIdustryforSelenScieneandEngeing，WuhanPolytecicUiversitNtioalR&DCenterforSRichAgcral Products Procesing/HubeEngeigandTecnicalResearchCenterforDeepProcessingofGnSelen-RichAgriulturalProduts， Wuhan 430048，China）

Abstract：Toeteutilzcrorganismsfrenvronmentaloremediation，educeseleumpolutionisoil，andrationalyeplit seleniumresources，aselenumtolerantstrain，ZJ2，wassolatedfromselenum-richsoilinYutangba，EnshiCity，HubeiProvice. MolecularbiologicalidentificationconfimeditasBacilluscereus.Tisstrainexhbitedhightoleranceandreductioncapabilitytosodium selenite Na2SeO3 ），converting itinto stable biogenic selenium nanoparticles（SeNPs）.Additionally，theeffctsoffourdifferent carbonsourcesonthesodiumselenitereductioneficiencyandthestabilityofsynthesizedSeNPswereinvestigated.Theresultssowed that strain ZJ2 had a 92% reduction capacity to 5mmol/L sodium selenite.The bio-nanoselenium synthesised by this strain was sphericalparticleswithanaverageparticlesizeof（188.422.63）nmandaZetapotentialof（-41.17±1.77）mV.Itwasfoundthatheadditionof rhamnoseand glucose promotedthereductionofsodium selenitebystrain ZJ2，withan increase inthereductionrateof 37.48% （204号 and 39.53% ，respectively.Inaddition，rhamnose hadagood promoting effectonthe synthesisof stable seleniumnanoparticles bythe strain.Basedonacomprehensiveevaluationof thesodiumselenitereductioncapability，characterizationofseleniumnanoparticles （SeNPs）andtheirstabilityitwasdemonstratedthattheditionofhamossignificantlyenhancedtheabiltofBcilusceresZ to efficiently synthesize more stable biogenic SeNPs.

KeyWords：selenium-resistant strain；Baciluscereus；nano-selenium；reductionrate；stability；carbon source

微量元素硒（Se）是一种非金属元素，是在1817年由瑞典化学家永斯·雅各布·贝采利乌斯发现并命名的[。（剩余14835字）