桑寄生种子萌发过程中的生理生化变化

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关键词:桑寄生(Taxilluschinensis);种子;果胶;萌发过程;生理生化指标

中图分类号:S567.19;Q945.34 文献标识码:A

文章编号:0439-8114(2025)05-0083-07

DOI:10.14088/j.cnki.issn0439-8114.2025.05.013 开放科学(资源服务)标识码(OSID):

Physiological and biochemical studies on germination dynamics of Taxillus chinensis seeds

FENGChang-cun1,LIANG Lu2 ,LIANGWen-jing³,GUILing-jian³,SONGLi-sha³, PAN Li-mei,FU Jin- e3 ,WANLing-yun³,WEI Shu-gen1.3

(1.SchoolofPaacyuagieicalUvesityainoina;GradateClgangiUiersityfseii

Naning53O2O,China;GuangxiBotanicalGardenofMedicinalPlants/GuangxiKeyLaboratoryofHigqualityFomatioandUtilaif Dao-di Herbs,Nanning 530023,China)

Abstract:UsingTaxiluschinensisseeds withpectinasthecontrolgroupandde-pectinatedseedsas thetreated group,samples were collected at 0 ,5,10,15,2O,and25daysofcultivation.Thenrelevantphysiologicalandbiochemical indicators were determinedand comparativelyanalyed.Theresultsshowedthatthepolysaccaridecontentinthecontrolexhibitedaniitialincreaseandtefollowedbyadecrease,whilethede-pectinated treatmentshowedacontinuousdecliningtrend.Thesolublesugarcontentinbothgroups displayedadecreaseincrease-deceasepater,withsignificantlyowerlevelsobseredinthd-pectiatedtreatmentcoparedto thecontrolthroughout thegerminationpross.TheMDAcontentinthecontrolgroupshowedanupwardtrend,hileinthedepectinatedgroup,adownward-upward-downwardtrend was exhibitedand itwas mostlylowerthanthatinthecontrolduring germination. Thecontent of H2O2 and 02- showed similar trends in both groups,but thecontent inthede-pectinated group was mostly higher than thatinthecontrolduringgermination.TheactivitiesofSOD,ODandPALexhibitedsimilartrendsinbothgroups.TheSODactivity inthede-pectinatedgroupwasconsistentlyhigherthanthatinthecontrol,whilePODandPALactivities,lthoughlowerhanthose in the control,showed a continuous upward trend. The endogenous hormone content (IAA,ABA, GA3 ,ZR)exhibited fluctuating changesinbothgroups,withthecontrolgroupshowingageneraldowwardtrend,whilethede-pectinatedgroupshowedasignificant increaseandageneralupwardtrend.Itwaspreliminarilyconfirmedthatpectinremovalcouldpromotetherapidmobilizationoftored substances,enzymesandendogenous hormones intheseedsofTaxiluschinensis,promoting thesmooth germinationofseeds.

KeyWords:Taxillus chinensis;seed;pectin;germinationprocess;physiological and biochemical indicators

桑寄生(Taxilluschinensis)属于桑寄生科钝果寄生属植物,主要分布于广西、广东、云南、四川以及贵州等地,是传统的常用大宗中药材。(剩余10092字)

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