龙牙草多酚提取工艺及其抗氧化活性研究

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关键词：龙牙草（AgrimoniapilosaLedeb.）；多酚；提取工艺;抗氧化活性

中图分类号：R284.2 文献标识码：A

文章编号：0439-8114（2025）05-0128-06

DOI：10.14088/j.cnki.issn0439-8114.2025.05.020 开放科学（资源服务）标识码（OSID）：□

Study on the extraction process and antioxidant activity of polyphenols from Agrimonia pilosa Ledeb.

LIMing-zhu1，YUANLei'，ZHANGXue-zhen1，YANXiao-qian² （1.School of Culinary Arts，Qingdao Preschool Education College，Qingdao 266300，Shandong，China； 2.School of Food Engineering，Qingdao Institute of Technology，Qingdao 2663Oo，Shandong，China）

Abstract：ToimprovetheextractionrateofpolyphenolsfromAgrimoniapilosaLedeb.，liquid-to-materialratio（A），ultrasonic time （B）， SHMP addition （C）， and ultrasonic power （ D ）were selected as influencing factors based on single-factor experiment results.Afour-factorthree-levelresponsesurfacemethodologywasdesignedwithpolyphenolyieldastheresponsevaluetooptiizethe extractionprocess，andtheantioxidantcapacityofthepolyphenolsasdeterined.Theresultsshowedthateoptimalextractioconditions for polyphenols from Agrimonia pilosa Ledeb.were the liquid-to-material ratio of 25：1 （ mL/g ），ultrasonic time of 40 min，SHMP addition of 0.5% ，and ultrasonic power of 16O W，with a polyphenol yield of 4.35% under these conditions.When the concentration of polyphenols from Agrimonia pilosa Ledeb.ranged from 0.1 to 1.5mg/mL ， the maximum scavenging rates for superoxide anion radicals，DPPH radicals，and hydroxyl radicals were 59.80% ， 43.61% ，and 32.72% ， respectively. The scavenging capacity increased with polyphenolconcentration，demonstrating the antioxidant abilityof Agrimonia pilosa Ledeb.polyphenols.

Key Words：Agrimonia pilosa ledeb.；polyphenols；extraction process；antioxidant activity

龙牙草（AgrimoniapilosaLedeb.）为双子叶蔷薇科多年生植物，《本草图经》记载其全草可用于止血解毒。（剩余6009字）