烤烟K326的NtALS基因单碱基编辑与抗除草剂材料创制

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关键词：乙酰乳酸合成酶；烤烟K326；除草剂；基因编辑

中图分类号：S572 文献标识码：A

文章编号：0439-8114（2025）05-0102-03

DOI：10.14088/j.cnki.issn0439-8114.2025.05.015 开放科学（资源服务）标识码（OSID）：口

Tobacco's NtALS gene editing to develop herbicide-resistant new elite of K326

SUN Xiao-qiong1，2，ZHANG Xiao-lian³，GUO Yu-shuang³，HUJian-lin1，HUPeng1，ZHENG Xing-fei1，，WANG Hongbo1，XUE Lian1，XU De-ze1， ZHONG YU-ping²，YIN De-suo1，WANG Feng² （1.InstituteofFoodCrops，HubeiAcademyofAgriculturalSciences，Wuhan43064，China;2.Hubei HuichuzhiBiotechCompany，Wuhan 430064，China;3.Guizhou Academy of Tobacco Science，Guiyang 55oo81，China）

Abstract： In order to breed new varieties of flue-cured tobacco resistant to herbicide，the NtALS gene of flue-cured tobacco K326 was edited by the single base，the 194th proline triad code CCA of tobacco NtALS gene was replaced with leucine encoding CTA and TTA. Sequencing results showed that the editing rate was up to 20.69% ，the identification results of the herbicide spraying resistance of positiveedited plantsshowedthatheresistancewasobvious，andanewflue-curedtobacco materialwithherbicideresistance was developed.

Key words：acetolactate synthase（ALS）；tobacco K326；herbicide；genome editing

乙酰乳酸合成酶（AcetolactateSynthase，ALS）是存在于几乎所有植物生长中的质体酶，也存在于一些细菌和酵母中。（剩余4168字）