小麦粉中4种黄曲霉毒素的高效液相色谱检测方法学建立与评价

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中图分类号 S-3 文献标志码A 文章编号 1007-7731（2026）07-0096-05

DOI号10.16377/j.cnki.issn1007-7731.2026.07.024

Establishment and evaluation a high performance liquid chromatography method for the determination four aflatoxins in wheat flour

Du Meng1LiWenwen1 Liang Weihong²Wang Xiaojun1JiFei³Huang Yichun4 Jia Ruijie1Wang Luying1Sun Yunchao1 （ ， 252ooo，

² Ofice， Dongchangfu District， 252O0o，

3 Development Service Center Chiping District， 2520Ooo，

4Linqing City Bureau Agriculture and Rural Affairs，Linqing 2526Oo， China）

Abstract To establish an efficient method for the determination aflatoxins B1 ， B2 ， G1 ，and G2 （AFB 11 ，AFB2，AFG1， AFG 2 ） in wheat flour，this study systematicallyoptimized sample pretreatment and chromatographic conditions basedon high-performance liquid chromatography with fluorescencedetection （HPLC-FLD）.Theresults showed thatacetonitrile一 water（80：2O，v/v）combinedwith sodium chloride salting-out as the extraction solvent significantly improved the recovery targetanalytes.After purification with immunoafinitycolumns and derivatization with trifluoroacetic acid， matrix interferences were effectively removed.Under the optimized chromatographic conditions，the four aflatoxins exhibited good linearity in the concentration range 0.1-20.0ng/mL ，with correlation coefficients （r2） all greater than 0.999 9.Method validation results indicated that the limits detection （LOD）ranged from 0.008 to 0.017ng/g ，and the limits quantification （LOQ）were 0.026-0.055ng/g .Atspiked levels 0.5-5.0ng/mL ，theaverage recoverieswere 80.2%-89.5% ，with relative standard deviations （RSDs） all less than 2.0% . The RSD the six-replicate stability test was below 1.5% ， and the RSD for quality control sample analysis was less than 0.12% ， demonstrating good method stability.Inconclusion，thedeveloped method featuredsimple pretreatment，highsensitivity，andsatisfactoryaccuracy， and was suitable for the trace determination multiple aflatoxins in wheat flour.

Keywordsaflatoxins；pre-column derivatization；immunoaffinity column cleanup;high performance liquid chromatography; wheat flour

黄曲霉毒素（AFT）被世界卫生组织（WHO）国际癌症研究机构列为I类致癌物，具有毒性强、污染范围广等特点，我国明确规定粮食中黄曲霉毒素 B1 （ AFB1 限量值为 5μg/kg[1] 。（剩余5781字）