交叉引物扩增法检测食品中大肠杆菌不耐热肠毒素的探讨

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摘 要：CPA技术与常规PCR技术相比，既避免了对昂贵的仪器设备的依赖性，又能减少检测费用，且由于其特异性高、灵敏度高，可大幅度提高检测速度。本文基于交叉引物扩增法技术及应用，设计了使用该方法检测食品中大肠杆菌不耐热肠毒素的实验，以期能够为相关人员提供借鉴与帮助。

关键词：交叉引物扩增法；大肠杆菌；不耐热肠毒素

Detection of Heat Labile Enterotoxin of Escherichia coli in Food by Cross Primer Amplification

LI Wei， REN Zhongjie

（Yuncheng County Inspection and Testing Center， Heze 274700， China）

Abstract： Compared with the conventional PCR technology， CPA technology can not only avoid the dependence on expensive instruments and equipment， but also reduce the detection cost， and because of its high specificity and sensitivity， it can greatly improve the detection speed. Based on the technology and application of cross primer amplification， this paper designed an experiment using this method to detect heat labile enterotoxin of Escherichia coli in food， in order to provide reference and help for relevant personnel.

Keywords： cross primer amplification; Escherichia coli; heat labile enterotoxin

近年来，由于食品行业的迅速发展，食源性致病微生物引发的食物中毒、食源性疾病的发生率不断增加，给人们的健康和生活带来极大的威胁，同时也给我国的公共健康和经济带来了巨大损害。（剩余3761字）