利用InDel分子标记鉴定新乡小包23大白菜种子纯度

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The seed purity identification of Chinese cabbage Xinxiangxiaobao 23 by InDel molecular marker

XUShijing'， WANG Xiaoling1， XIAO Yan'， YUANRanghua'， YANG Chen²， JI Keying² （1.Xiniag Henan，China）

Abstract：Torapidlydetectthepurityofhybridseedsfromfrozendamaged plots intheoverwinteringseed production base of Xinxiangxiaobao23，ensure that the qualityof seeds entering the market meets standards，protect consumers rights，ad improvethe eficiencyofhybridseed screening，specific primers were selectedfrom12 pairsof InDelprimers for Chinesecabbage to distinguish Xinxiangxiaobao 23andits parents，and seed identification wascarriedout.The resultsshowedthatthree pairsof primersexhibitedco-dominance，amongwhichonepaircould effectivelydistinguish Xinxiangxiaobao23fromtheotherthreesimilarvarieties.This primerwas verifiedin108hybrid progenies，andthe purity identification result was 93.5% ，whichwas basically consistentwith the field identification result.In conclusion，the InDelmarkerBrID9ol05selectedinthis studycanbeusedforrapid identificationof seedpurityin Xinxiangxiaobao 23， promoting the healthy development of the seed industry.

Key words：Chinese cabbage;Xinxiangxiaobao 23;InDel molecular marker; Seed purity identification

大白菜（Brassica rapaL.ssp.pekinensis）属于十字花科芸臺属植物，在我国广泛种植，年种植面积多达180万 hm2 ，北方地区，如山东、河北、河南等地成为大白菜的主产区。（剩余7247字）