柿砧“苏砧1号”组培快繁体系的建立

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关键词苏砧1号;柿;组织培养;叶片再生中图分类号S665.2文献标识码A文章编号 0517-6611（2026）01-0062-05doi：10.3969/j.issn.0517-6611.2026.01. 012开放科学（资源服务）标识码（OSID）：

lapidPropagation of Persimmon Rootstock“SuzhenNo.1”by Tissue Culture

YUJiang-tao，WUXu-ting，HUDa-guietal（CollgeofHorticulture，NanjngAgricultural University，Nanjing，Jiangsu2095） Abstract [Objective]Arapid propagationsystem for“Suzhen No.1”wasestablished through tisueculture techniquesandleaf-induced regeneration，providingatechcalfoundationforislargescaleadficientmiplicatioMetod]Domantudsof“SuenNo”wee usedasexplantsforpriarytisueculture，andtheirgrowthstatuswerecompaedtoconfimtheostsuitableminimalmedium.Teoptial subcultureconditioswereidentfiedytestingcombinatiosofIAAandatvaringconcetratios.eotialhoronconditioforthe regenerationofleaveswereconfiedbycombingdiferentconcentrationsofDZandNAA.Result]（/2N）MSwasidentfiedaseotimal minimal medium for“Suzhen No.1"tissue culture. The dormant bud germination rate reached 76. 0% ，with vigorous plant growth and tender green leaves；subcultured shoots exhibited the best growth on（1/2N）MS + IAA 0.1 mg/L + ZT1.O mg/L，reaching an average height of 37.8mmafter3，withaproliferationcoefcietof4.6，igrconentrationofZ3.Omg/Lexeredanibioryeetoneowthf subcultured shoots；Leaf explants showed optimal regeneration on （1/2N）MS + TDZ 3.0 mg/L + NAA 0.2 mg/L，achieving acallus induction rate of 90.0% and an average of3.1adventitious shots per explant.[Conclusion]Arapid tisse culture propagation systemfor“Suzhen No. 1"was established： （1/2N）MS medium was the most suitable basal medium; （1/2N）MS supplemented with 0.1 mg/L IAA and 1.0mg/L （2号 ZT provided optimal conditions for subculture；（1/2N） MS supplemented with 3.0mg/L TDZ and 0.2mg/L NAA was optimal for leaf callus induction.

Key wordsSuzhen No.1;Persimmon;Tissue culture;Leaf regeneration

柿树是江苏省传统栽培的主要经济林果树树种之一，其产业发展方向正逐步转向商品价值高、市场需求旺盛的甜柿品种。（剩余8923字）