花椰菜小孢子培养及植株再生研究

打开文本图片集

关键词花椰菜；小孢子培养;双单倍体;再生植株;倍性检测

中图分类号S635.3文献标识码A

文章编号 0517-6611（2026）01-0058-04

doi：10.3969/j.issn. 0517-6611. 2026.01. 011

开放科学（资源服务）标识码（OSID）：

tudyonCauliflowerMicrosporeCultureandPlantRegeneration

TAO LeiYUANQing-yun，YANGWen-jie（SchoolofHorticultureandForestry，HuazhongAgricultural Universit/National KeyLaboratory of Fruit and Vegetable Horticultural Crop Germplasm Innovation and Utilization，Wuhan，Hubei 430070）

AbstractOjectie]Tosudythefctosetigfrcrospeculurandplantregenerationoflflowerofdiferentmatuitytype， andptimizecauliflowricrosporeculturesystemtobtainregeneatedDHplants.Metod]Elevencauliflowrvarietiswithfoueet maturitytypselyaly-latelatetuityeeddtsofloplod spores，genotyps，ndtemperaturetreatmentsonthebrymergenceatewereaalyzed.Resut]ostoftemicrosporsibdswith lengths of 3.5-<4.0 mm were concentrated in the mononuclear marginal stage；there were great diferences in embryo emergence among differentgenotypsdvengeotsoutofteateralsaiedbrositmbromegenceateof6.4%；indo coldtreatmentandattreamentcouldprootetevelopntofcrosporembs，speciallthbrergeceratfalilower with the genotype of Xueyu 8O after 48h of cold treatment，which could reach 1.5O embryos/bud.Inaddition，both cold treatment and heat strestreatmntuldprootetevelopntofcrospebsspeciallbpoducoateofulifowriteX eyu 80 after 48h ofcold treatment could reach 1.50 embryos/debut.[Conclusion]Genotype is a decisive factor in determining whether embryoscanbeproducdfrocauliforicrosporeculure，teengthofflowerbudsdeteinesteperiodofcrospordevelopntdte timeof coldtreatment and shock treatment hasacertain effectontheembryo productionrateofcauliflower microspores.

Key WordsCauliflower;Microspore culture ;Double haploid ;Regenerated plants ;Ploidy detection

花椰菜（Brassicaoleraceavar.botrytisLinnaeus）又名菜花、花菜，是十字花科芸墓属甘蓝变种二年生草本植物。（剩余7920字）