甲基磺酸乙酯（EMS）诱变对辣椒种子萌发及幼苗生理特性的影响

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中图分类号：S641.3 文献标志码：A 文章编号：1673-2871（2025）12-196-07

Effects of ethyl methanesulfonate（EMS）mutagenesis on seed germination and seedling physiological characteristics of chili pepper

Abstract：Toidentifytheoptimal treatment conditions for ethyl methanesulfonate（EMS）mutagenesisofchili pepper seeds，this experiment used the founder parent line O135of Pingjiao pepper as material.Employing both petri dish germinationandnurserytraysedlingcultivationmethods，withdistilledwaterasthecontrol，thestudy investigatedtheeffects of16-hour seed soaking treatments with different EMS concentrations 0.7% 0.8% 0.9% 1.0% 1.1% 1.2% ，and 1.3% ） on chili pepper seed germination（germination potential，germinationrate，germination index），seedling emergencerate， seedling establishment evaluation indices（plant height，stem thicknes，rootlength，fresh and drymass of aboveground and underground parts，photosynthetic pigmentcontent）as well asMDAcontent，andPOD，CAT，and SODactivity in the leavs.Theresults indicated thatEMS treatment significantlyinhibitedchili pepper seed germinationandseedling emergence.Thegermination potential，germinationrate，germinationindex，andsedlingemergenceratealldecreased with the increasing EMS concentration.At an EMS concentration of 1.1% ，the seed germination rate （45.33% ）and seedling establishment rate 42.27% ）were closest to the semi-lethal dose.EMS mutagenesis significantly reduced the content of photosynthetic pigments in seedlings and significantly increased the MDAcontent inseedling leaves.The increaseof MDA content was positively correlated with EMS concentration.When the EMS concentration Was less than 1.2% ，itpromoted the increase of SOD，CAT，and PODactivity inseedling leaves.Atan EMSconcentrationof 1.3% ，it still promoted CAT activitybutinhibitedSODandPODactivity.Inconclusion，undertheconditionsof this experiment，anEMSconcentration of 1.1% is the optimal treatment for chili pepper mutagenesis.

Keywords：Pepper;EMS;Mutagenesis; Seed germination; Seedling physiological characteristics

棘椒为一年生或多年生草本植物，是全球消费量最大的辛辣调味品[，是具有显著品牌优势的重点产业[2。（剩余10411字）