单粒芹菜干种子基因组DNA快速提取方法的建立

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中图分类号：S636.3 文献标志码：A 文章编号：1673-2871（2025）12-071-06

Establishment of rapid genomic DNA extraction method from single dry celeryseed

WU Feng'，LI ， WANG Wutai'，HUA ，GAO

（1.InstituteofVgetablesnjincdeyoficltualecetateKeyLbratoyofVegetableobding92

China;2.choolofifeiencesnjinUiversitynjino72，ia）

Abstract：GenomicDNAextracteddirectlyfromplantseedscouldbeusedformolecularidentificationofseedpurityand specific genotypes.There is no report on effcientdirect extraction methods for genomic DNA from single drycelery sed untilnow.Inthisstudy，thesingledriedceleryseed wassubjectedtoextractgenomeDNAbyalkaline lysismethod， which wasutilizedasthe template forPCR（polymerase chainreaction）amplification withActin-specific primers to verify whether theextractedgenomicDNAmets therequirements formoleculardetection.Theresultsshowedthatclearspecific bands wereobtained byPCRamplification，demonstratingthatthegenomic DNAdirectly extracted fromsingle drycelery seed was suitable for PCR amplification.To assess DNAstability，PCR amplification was conductedusing the genomic DNA stored at 4∘C for14 days，and successful amplification was confirmed by the presence of expected bands in agarose gels.Thisstudyestablishedarapid，simpleandhighlyefficient methodforextractinggenomicDNAfromindividualdried celeryseed.Theextracted genome DNAwassuitableforPCRamplificationand exhibited good stability.Itcouldbeused forMAS ofcelery seed purity and specific genotypes.

Keywords： Celery;Genomic DNAextraction;Single seed;Molecular marker assisted breeding

芹菜（ApiumgraveolensL.）属伞形科芹属草本植物，起源于南欧及地中海沿岸，是世界范围内广泛种植的重要叶菜类蔬菜，也是我国最重要的蔬菜之一。（剩余8654字）