高产IAA芽孢杆菌的筛选、鉴定及其对番茄的促生效应

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中图分类号：S641.2 文献标志码：A 文章编号：1673-2871（2025）12-171-07

Screening， identification， and growth- promoting effects on tomato of high-yield IAA-producing Bacillus

Abstract：Toscreen Bacilusstrainwithhigh Indole-3-aceticacid（IAA）production from therhizosphereofsweetcherries， clarifytheirgrowth-promotingcharacteristicsuchassiderooresecretion，phosphatesolubilation，potassiumsolbilization，andnitrogen fixationas wellas their growth-promoting efects，soas to provideabasis forthesubsequent developmentof microbial fertilizers forsweetcherry.Spectrophotometry wasusedtodeterminetheIAAproductionofplantrhizospheregrowth-promoting bacteria（PGPR）.Strain identification was conducted throughmorphologicaland16SrRNA sequence analysis.Qualitative detection wasperformedonthe growth-promotingcharacteristicsofthe strains including phosphate solubilization，potassium solubilization，andnitrogen fixation.Through the germination experimentof themodel plant，tomato，the growth-promoting efectofstrainsonplants was verified.Theresultsshowed thatfourhigh IAA-producingBacillus strainswere screened out，with IAA production ranging from14.79 to 20.71mg⋅L-1 .Theywere identified as Bacilusvelezesis（A81），aciluspaiicus（E2），Bacillusznthoxyli（15），ndcillsbus（R42），espeivly growth diameter of all strains ranged from 0.61 to 0.77cm ，and all had the abilities of acid production，siderophore secretion，organicphosphatesolubilization，potassiumsolubilization，andnitrogenfixation.Allstrainshadsigniicant growth-promoting effects on tomato seedlings，with the growth-promoting effects in the order of R42>S151> A81 > E2. Compared with thecontrol（CK），R42and S151 significantly increased the plant height by 26.39% and 20.00% ，respectively，while their root length significantlyincreased by 17.51% and 16.61% ，respectively.In conclusion，the rhizosphere of sweet cherryharbors multifunctional growth-promotingBacillus with highIAA production，and theresultsof this study provide important bacterial resources for the development and applicationof functional microbial fertilizers.

Keywords：Tomato;Rhizosphere bacillus; IAA; Growth-promoting effect

植物根际促生菌（PGPR）是指生活在根际土壤或依附于植物根系的一类有益微生物，能促进植株养分吸收及生长，抵御不良环境，提高植物适应性[-2]。（剩余11931字）