美洲南瓜全基因组InDeI标记的开发与应用

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Genome- wide InDel marker development and application of Cucurbita pepo L.

DONG Jiamei1， YANG Yongzhao，SUN Liuqing，LU Qiuchen CHEN Wanlu，WU Zhe'，DUAN Ying，LEI Fengjin，WANG Changlin，BOKailiang

（1.ColegeoforictureaiicultualUvesityginastiteofVegetablesdowe ChineseAcademofAicturalentateKybatoyfVegetablebdineiioisito Research，hanxiAgriculturalUniversity，YunchengO44ooo，hanxi，China）

Abstract：Pumpkin（CucurbitapepoL.）isanimportantvegetablecropswithrichgeneticdiversity，diverseuse，and high economicvalue.Tosystematicallydevelopgenome-wideInDelmarkers，twoaccessions fromdifferentsubspecies-Jin-GL （C.pepo ssp.texana）andHM-S2（C.pepo ssp.pepo）-were subjected toresequencing.Five polymorphicInDel locis were selected and successfullyvalidatedoneach chromosome，resulting inatotalof10 pairs primers.Polymorphismevaluationusing81pumpkingermplasmsrevealedbroad geneticbackgroundsamong thetestedmaterial，withanaverageShannon's genetic diversityindex of1.0o，andhighmarker polymorphism，as indicatedbyanaverage polymorphismiformationcontent（PIC）of0.56.Clusteranalysis viatheunweighted pair group method with arithmetic mean（UPGMA）classifiedthe81 germplasms into four gourps atagenetic similaritycoefficientof0.65.GroupIcomprised45accessions，primarilyvining ormamental typesofsp.texana.Group IIcontained34accessions，representingcultivated types ofsp.pepo. Group IIl contained one wild accession of C.foetidissima and group IVconsistedofone C.maxima accession.Ten markerswith thehighestpolymorphismwereselectedfrom10Opolymorphic InDelmarkers toconstructafingerprinting.Finally，clusteranalysiswasperformedon237commercialC.pepovarieties（andhybrids）usingthe10Opairsofprimers.The results showed thatatthe genetic distanceof0.08did seed-types exhibit a tendencyto formadistinct group.Furthermore， mostvarieties （hybrids）ofboth flesh-typeandseed-typeexhibitatrendofnarrowgeneticbackground.Thisstudyprovides atheoretical basis for the genetic diversity analysisof C pepo germplasm resources and the development of new cultivar. Key words： Cucurbita pepo L.; InDel marker; Genetic diversity; Fingerprinting

我国是南瓜生产与消费大国，播种面积和产量均居世界前列，2023年我国南瓜栽培面积约40.11万 hm2 ，产量约742.33万t，分别占世界总量的 25.84% 和 31.35% ，南瓜栽培面积和产量均居世界第一位（FAO）。（剩余9810字）