甜瓜叶片三种DNA提取方法对KASP标记检测体系适用性的研究

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中图分类号：S652 文献标志码：A 文章编号：1673-2871（2025）12-030-07

Comparative study on the applicability of three DNA extraction methods frommelon leavesforKASP markerdetectionsystem

LI Wulin'，MA Jiaxin， HE Yuhua， TANG Lingli'，XU Yongyang，HUANG Xiang， ZHAO Guangwei （1.Zhengzoechedecalooi; searchCenter，hineseAcademyofAgriculturaliences，iniang4535oo，Henanina）

Abstract：To investigateDNAextractionmethods frommelon leavessuitableforKompetitiveAlele-Specific PCR （KASP）marker detection system，this study used melon parents B551，B22 and their F1 hybrid B551×B22 ）asmaterials.Three DNAextractionmethods includingalkaline lysis，CTABandacommercial magnetic bead-based kit-were employed to extract DNA from melon leaves.The efects ofthese three DNA extraction mehods on the KASP marker detectionsystem wereanalyzed.DNAqualityassessmentresultsshowed thatthecommercial kit method efectivelyremoved impurities such as proteins，polyphenols，and polysaccarides from melonleaves，achievingaKASPdetectionaccuracyof 100% .TheCTABmethodachievedadetectionaccuracyof 97.2% ，whilethealkalinelysismethodachievedadetectionof 69.4% . In conclusion，thecommercial magnetic bead-based kitis the mostsuitable for high-throughput KASPdetection， the CTAB methodcanbeused forsmal-scaledetection，andthealkalinelysis methodisnotsuitableforDNA extraction from melon leaves in KASP marker detection systems.

Keywords： Cucumismelo; KASP genotyping;DNA extraction method

甜瓜是葫芦科黄瓜属一年生草本植物，有着悠久的栽培历史，它在我国果蔬产业和消费市场中占有重要位置[-2。（剩余9002字）