马铃薯StWRKY70基因启动子功能分析

打开文本图片集

Functional analysis of the StWRKY70 gene promoter in potato

GENG Deying， GUO Yuheng， LIU Shipeng， ZHAO Yaqian， ZHAO Dongmei， LI Qian， YANG Zhihui （Collegeotoeioniiculuralityoinei）

Abstract：The transcription factor StWRKY7Oisinvolved inplant diseaseresistancedefenseresponsesandcanenhance potato early blight resistance.To investigate the promoter function of the potato StWRKY70 gene promoter，this study clonedthe promotersequenceusingPCR methodand predictedits cis-acting elements usingonlinetoanalyzethedistributioncharacteristicsofregulatoryelements.Aditionall，5'-enddeletion expression vectorofthepromoter（pGreenI 0800-StWRKY7Opro：LUC）were constructed.The expression of thereporter gene LUC wasobserved through transient transformation of tobacco leaves to identifythe promoter’sactiveregions.Theresults showed that the length of the promoter sequencewas2ooo bp，andcontainedvarious stres-responsivecis-elements，suchasdehydration-responsiveelementMYB，drought-responsiveelementAP2/ERF，andhigh-temperature-responsive elementHSF.Theanalysis resultsof LUCfluorescence intensity showed that with the increase of the length of different 5′ -end deletion fragmentsof the St WRKY70 gene promoter，the LUC fluorescence intensitygradually increased，indicating thatthecoreregionof the St. WRKY70promoterwas 400bpto70o bp.Inconclusion，thecoreregionandvarious stress-responsive cis-acting elements ofthe StWRKY70 gene promoter may be involved in the potato's defense response against early blight byregulating gene transcriptioneficiency，therebyprovidingcertaintheoreticalsupportforfurtherelucidatingthemechanismunderlying the role of StWRKY70 in plant disease resistance.

KeyWords：Potato;Promoter activity; StWRKY7o;LUC fluorescence intensity;Tobacco conversion

马铃薯早疫病是由茄链格孢（Alternariasolani）引起的一种真菌性病害，主要危害马铃薯的叶片，是仅次于马铃薯晚疫病的第二大病害[1-2]。（剩余11429字）