高效液相色谱法测定梨中乙氧喹啉的残留量

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Determination of Residual Ethoxyquinoline in Pear by High Performance Liquid Chromatography

SU Yangbo， JIANG Xinhui， WANG Bing （ ，， ， ）

Abstract： Objective： To compare two HPLC methods for the determination of ethoxyquinoline residue in pear. Method：The samples were extracted with n-hexane under alkaline condition，dried by rotation，and determinated with acetonitrile containing ascorbic acid. High performance liquid chromatographic fluorescence detector （HPLCFLD）and high performance liquid chromatographic tandem mass spectrometry （HPLC-MS/MS） were used for the determination. Result： Ethoxyquinoline showed a good linear relationship in the range of 2μg⋅L-1 to ，and the correlation coefficient was greater than 0.999.The limits of detection and quantification of HPLC-FLD were 10μg⋅kg-1 and 20μg⋅kg-1 ， respectively. The limit of detection and limit of quantitation of HPLC-MS/MS method were 0.5μg⋅kg-1 and 1.0μg⋅kg-1 ，respectively. Conclusion： HPLC-MS/MS method has high separation efficiency and high sensitivity，and issuperior toHPLC-FLDmethod inthe determinationofcomplex substrates.Thedetectioncostof HPLC-FLD method is lower than thatof HPLC-MS/MS.Therefore，the appropriate detection method canbe selected according to the detection requirements.

Keywords： high performance liquid chromatographic; pear; ethoxyquin

食品农药残留超标不仅会对人体健康造成危害，还可能破坏生态平衡。（剩余3222字）