聚多巴胺功能化普鲁士蓝用于黄曲霉毒素 B₁ 适体传感研究

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Polydopamine-Functionalized Prussian Blue for Aptamer Sensing of Aflatoxin B1

JIANG Ziping， CHEN Jiajie，BIN Xin， ZHAO Qian*， SHI Xingbo （College ofFood Science and Technology，Hunan Agricultural UniversityHunan Provincial KeyLaboratoryofFood Science and Biotechnology， Changsha ， China）

Abstract： This article improves the traditional enzyme-linked immunosorbent assay and establishes a novel immunoassay technology based on enriched polydopamine Prusian blue nanoenzyme and adapter binding for high-sensitivity detection of aflatoxin B1 （AFT B1， ）. The results showed that the immunoprobes functionalized with polydopamine and binding to adapter Prussian blue were successfully synthesized; under the optimal reaction conditions， the regression equation for the AFT B1 colorimetric signal is A652nm=0.101gc（AFTB1）+0.72 ，with a detection limit of 0.22pg⋅mL-1 ， verifying the good specificity of this method; In the detection of AFT ΔB1 in rice samples， the recovery rate ranged from 96.41% to 111.19% ，indicating that this method has broad application prospects in the detection of food AFT B1 ：

Keywords： aflatoxin B1; Prussian blue; aptamer; enzyme-linked immunosorbent assay

黄曲霉毒素是一类主要由黄曲霉和寄生曲霉产生的次生代谢产物，具有致突变性、致畸性和致癌性，极易对大豆、花生和玉米等农作物及其加工产品造成污染，是对人类健康威胁最大的霉菌毒素之一。（剩余8938字）