高效液相色谱法测定乌鸡中氟喹诺酮类药物残留量

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Detection of Fluoroquinolone Residues in Black-Boned Chicken Using High-Performance Liquid Chromatography

HUANG Ruihong （Chongzuo Aquatic and Livestock Technical Service Center， Chongzuo 532200， China）

Abstract： A high-performance liquid chromatography （HPLC） method was established for detecting residues offourfluoroquinolones in black-boned chicken.Thechicken samples were minced，homogenized，andextracted using a phosphate bufer solution，followed by purification with solid-phase extraction columns.The analytes were then quantified via HPLC.Results showed that under optimal experimental conditions，the linear relationships for ciprofloxacin，danofloxacin，enrofloxacin，andsarafloxacin were excellent，with correlation coficients R2 ） exceeding 0.999. The method demonstrated detection limits of 0.474 6μg⋅kg-1 to 4.849μg⋅kg-1 and quantification limits of 0.943 6μg⋅kg-1 to 10.070μg⋅kg-1 for the four target analytes. At three spiked concentration levels， the average recoveries of the four fluoroquinolones ranged from 80.38% to 101.70% ，with relative standard deviations between 0.46% to 1.52% .These findings indicate that the method is accurate，sensitive，and reliable，meeting the requirements for veterinary drug residue analysis.It provides a technical reference for detecting restricted and banned fluoroquinolones in animal-derived food products at the grassroots level.

Keywords： high-performance liquid chromatography （HPLC）; fluoroquinolones; black-boned chicken; drug residues

乌鸡是广西食用农产品“治违禁控药残促提升”三年行动部署中11个重点治理的品种之一，容易出现氟喹诺酮类药物残留超标。（剩余3521字）