超高效液相色谱－串联质谱法测定火麻啤酒中大麻酚、大麻二酚和四氢大麻酚

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Determination of Cannabinol， Cannabidiol and Δ9 1 Tetrahydrocannabinol in Hemp Beer by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry

ZHU Faze12， CHEN Yuemeng， XIAO Dexin4， ZHAO Yuwang'， CHEN Shunqin1²， ZHANG Qiaojun1.2*

（1.Department or Forensic Medicine， Guizhou Medical University， Guiyang 55oo04， China; 2.School ofForensic

Medicine， Guizhou Medical University， Guiyang 55oo04， China; 3.Guiyang City Public Security Bureau Drug

Testing Center， Guiyang 550o08， China; 4.Guizhou Wanfu Xianyi Testing Technology Co.，Ltd.，Guiyang 550009， China; 5.School of Public Health， Guizhou Medical University， Guiyang 56l1o0， China）

Abstract： Objective： To establish a method for the determination of cannabinol （CBN），cannabidiol （CBD）， and Δ9 -tetrahydrocannabinol （THC） in hemp beer using ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）. Method： The samples were extracted with ethyl acetate and subjected to gradient elution with water （containing 0.25% formic acid and 0.2% ammonium formate） and acetonitrile. Separation was performed on a Waters ACQUITY HSS T3 column. Detection was carried out using electrospray ionization in multiple reaction monitoring mode， with quantification by external standard method.Result： CBN，CBD，and THC showed good linear relationship in the range of ，and the correlation coefficient r value was greater than O.999. At the three addition levels of 1， 10ng⋅mL-1 and 100ng⋅mL-1 ，the sample recoveries were （20 72.9%～106.4% ， the matrix effect was -2.5%～11.3% ， the accuracy was -21.1%～-9.8% ， In the relative standard deviation （RSD）， the intra-day RSD was 2.3%～11.2% ，theinter-day RSDwas 3.5%～14.2% ，the detection limit was 0.5ng⋅mL-1 ，and the quantification limit was 10ng⋅mL-1 . Conclusion： This method is simple， rapid， sensitive， andrequires lessreagents in the pretreatment processItcan provide reliable technical support for the identification of illegal cannabis components in food safety supervision and public security drug enforcement.

Keywords： ultra-high performance liquid chromatography-tandem mass spectrometry; cannabidiol; cannabinol; Δ 9-tetrahydrocannabinol; illegal addition

火麻，属桑科大麻属，为一年生直立草本植物，其起源可追溯至锡金、不丹、印度及中亚等地区。（剩余8524字）