一株酸性果胶酶产生菌的筛选及酶学特性分析

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Screening and Enzymatic Characterization Analysis of an Acid Pectinase-Producing Bacteria

FUJinling,MA Mengyao,LANWeishen, ZENGLiqing,SUNJiaman,LI Shuai* (School of Chemistry and Environmental Science, Xiangnan University, Chenzhou 423oo0, China)

Abstract: In this paper, a strain of pectinase-producing bacteria was screened from the soil by Congo red method,and the enzymaticcharacteristics ofthe pectinase produced by pectin were studied.The results showed that afilamentous fungus with pectinase-producing ability was screened from the soil,and it was identified as Aspergilus niger after molecular biological identification. Under the conditions of 50∘C and pH=4.0 , the pectinase enzyme activity produced by this bacterium was the best. When the pH value is 3.0 to 5.0, its stability is good.

Keywords: pectinase; Aspergillus niger; enzymatic properties

果胶酶来源丰富,植物、动物和微生物均具有果胶酶合成能力。(剩余3934字)

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