应用响应面法优化马铃疮痂病生防链霉菌PBSH9发酵培养基

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中图分类号：S532 文献标志码：A 文章编号：1673-2871（2025）11-065-12

Abstract：Potatocommon scab isa significant disease that restrictsthe development ofthe potato industry.This study screenedthe biocontrolstrainPBSH9，andits metabolites showedsignificant inhibitoryefects onthepathogen，achieving a field control efficacy of 73.97% .To identify the active compounds，the secondary metabolites of strain PBSH9 were isolatedandpurified，sucessullyyielding theactivesubstance.Tonhance theyieldof thisactivesubstance，response surfacemethodology（RSM）was employed tooptimize itsculture medium.Basedonsingle-factor experiments and Placket-Burman tests，combined with the steepest ascent methodand Box-Behnken design response surface methodology to fit thenonlinearequationbetweensignificantfactorsandtheyieldofactivecompound，theoptimalfermentationmediumformula was obtained. The optimized formula was 4.50g⋅L-1 glucose， 50.00g⋅L-1 soluble starch， 15.00g⋅L-1 corn flour， 21.08g⋅L-1 soybean meal， 2.00g⋅L-1 peptone， 0.50g⋅L-1（NH）2SO4 0.60g⋅L-1MgSO4 ，and 0.04g⋅L-1KH2PO4 Themodel-predicted yield of active compounds was 199.92μg⋅mL-1 ，while the actual value was 184.35μg⋅mL-1 ，whichwas 5.83times higher than the yieldofcompounds inthebasicculture medium.This study providescrucialtechnical support for the development of efficient biopesticide products against potato common scab.

Keywords：Potatocommonscab;BiocontrolStreptomyces;Responsesurfacemethodology;Bioactivemetabolites;Medium

马铃薯（SolanumtuberosumL.）是一种历史悠久的粮食作物，在全球160个国家广泛种植，在农业生产中占据重要地位。（剩余17475字）