马铃薯StWRKYI5基因克隆与表达特征分析

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中图分类号：S532 文献标志码：A 文章编号：1673-2871（2025）11-026-10

Abstract：WRKY transcription factorsplayacrucialroleinthesignaltransductionpathwaysofplant diseaseresistance. Potato was selectedasthe modelorganism to investigatethe propertiesand functions of StWRKY15.RNA-seqanalysis identifiedone transcriptionfactor，StWRKY15，asbeingsignificantlyinducedbypotatoearlyblight.StWRKY15wassuccessfullyclonedusingPCRtechniques.Theful-lengthsequencespans1O02bpandencodes33aminoacids.Subcelular localisation was confirmed to bewithin the nucleus.StWRKY15 belongs totheunstable hydrophilic protein family.It has a molecular mass of approximately 33.6kDa andatheoretical pI of around 9.45.It exhibits the highest homology with CaWRKY15.Both proteinsaremembersof the WRKYfamily.AnalysisofStWRKY15expressionlevels invarious potato tisues usingqRT-PCRrevealed thehighest expression instems.The expression levelsof StWRKY15 were measuredat varioustimepointsafter inoculationwithAlternariasolani.TheresultsindicatedasignificantincreaseinStWRKY15expressionat24 hours post-inoculation，with peak expresionbservedat48 hours.This demonstrates that StWRKY15is stronglyinducedbypotatoearlyblight.TransientoverexpressionestablishedthatStWRKY15isassociatedwithenhanced resistance to Alternaria solani in tobacco.These results lay the groundwork for further research into the roleof St. WRKY15 inconferring resistance to early blight in potato.

Keywords：Potato;StWRKYi5;Bioinformatics;Subcelllar localization; Transient expression

马铃薯早疫病是一种由茄链格孢（Alternariasolani）引起的真菌性病害，主要危害马铃薯叶片，主要特征为产生明显的同心轮纹病斑。（剩余14022字）