不同基因型苦瓜幼苗耐低温鉴定及评价指标筛选

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Abstract：The efects of lowtemperature stressonphysiologyand biochemistryofMomordicacharantiaL.were studied to screenlow-temperature-tolerant materialsandevaluation indicators in41inbredseedlings.Theresultsshowedthatthe variationcoefficientof12physiologicalandbiochemical indicatorsamongthe41bittrgourdinbredlineswere 5% 150% ，which could be transformed into5 independent comprehensive indicators.The cumulative contribution rateof the main factors reached 74.22% .Correlation analysis indicated that Fv/Fm was significantly negatively correlated with MDA content and solublesugar content.The 41 different genotypes ofbiter gourd inbred lines could be clasified into 5 categories basedon the strength of low-temperature tolerance evaluatedbythe membership function method.Six inbred lines with relatively strong low-temperature tolerance were screenedout，and Pro content，SODactivity， Fv/Fm， MDA content and Ci were establishedas theevaluation indicators of low-temperature tolerance，which provides a relatively efficientandapplicablemethodfor theeficientandearly-maturingbreedingofbitter gourdandtheidentificationoflow-temperature tolerance materials.

KeyWords：Bitergourd;Lowtemperaturestress;Principal factoranalysis;Membershipfunction;Evaluationindex

苦瓜（MomordicacharantiaL.）起源于热带非洲的沙漠边缘，是适合在热带和温带地区及设施农业中生长的蔬菜和草本药用植物，富含维生素C和铁[，果肉及其种子还含有抗糖尿病和降糖化合物，作为一种食疗蔬菜深受中老年人的喜爱。（剩余9066字）