一测多评法同时测定五爪子中金丝桃苷·芸香苷和绿原酸的含量

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关键词五瓜子；一测多评法;金丝桃苷;芸香苷;绿原酸;相对校正因子

中图分类号R284文献标识码A

文章编号 0517-6611（2025）16-0175-05

doi：10.3969/j.issn.0517-6611.2025.16.038

AbstractObectie]Tstablishmetdofquantiativeanalysisofulti-ompoentsbygle-markrQAM）forsultaeoslyete mining the content of hyperoside，rutin，and chlorogenic acid in Wuzhaozi.[Method] Using Agilent HC- C18（2） chromatographic column （ 4.6mm×250mm ， 5μm ），with acetonitrile（A） -0.1% formic acid（B） as mobile phase，gradient elution，column temperature of 30qC ， flow rate of 1mL/min ，injection volume of 15μL ，detection wavelength of 360nm .Using chlorogenic acid as the internal reference，calculated therelativecorrectionfactor（RCF）betwnclorogicacidndhyperoside，rutin，andevaluatetedurabilityofCF；comparedadaalyzedtheresultsof theQAMSmethodwiththeexteraltandardmethod（ESM）.Result]Hypercintinandchlorogenicacidhadagd linear relationship within a certain range （ R2≥0.9988 ），and the system adaptability test，precision，repeatability，stability，and spiked recoveryratealletteeqremnts；tratoreonfactosfoperosidadutwereclaedt.9nd.esp tively，andthfctsofolutmperature，foate，ndjectionamoutonCFererelativelysallwitiertainrangethreas nosignficantdiferenceintemeasuementresultsbetweeQAMSndESM.Conclusion]TemetodofQAMSestablishedintisudycan beusedtodeterineteontentofhyperoside，utin，ndchlorogenicacidinWuzhaozi，andcanprovidereferencefortequalityevauation of it.

KeywordsWuzhoz;Metodofquantiativeaalyssofulticompoentsbysing-marker;Hyproside;utin;Chlrogenicacid;elative correctionfactor

五爪子为五加科植物刺五加［Acanthopanaxsenticosus（Rupr.et Maxim.）Harms]的叶，因其由五枚掌状小复叶组成而得名[1]。（剩余8255字）