应用小鼠血清学抗体替代靶动物评价口蹄疫疫苗效力

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关键词小鼠血清学抗体；靶动物；口蹄疫疫苗效力中图分类号S855.3文献标识码A文章编号 0517-6611（2025）16-0085-03doi：10.3969/j.issn.0517-6611.2025.16.018

AbstractObective]Indertoeducetheologicalsafetyrisks，ontrolthetectioncosts，ndshortenteevaluationperidoffotad mouthdiseasevacinfcacy.Method4batchsofiactivevacinesforOtypepigfotandmouthdiseaseproducedbChiaAltural Veterinary Biotechnology Co.，Ltd.were selected to detect their immune efficacy（ PD50 ）inpigsand the serum antibody level in immunized mice.The correlation between the above two indicators was also analyzed.[Result] The PD50 values of the 14 batches of vaccines ranged from 7.49to15.59，allofhichwerehghertantatofnatioaltandrdecorrelationcoefintsetweenteaverageantibdylevelsand PD50 in mice immunized with 1.0，0.5and 0.33mL doses were 0.89，0.95 and O.92，respectively.Among them，the correlation between the antibody level and PD50 was highest in the 0.5mL immunization dose group.[Conclusion]The feasibility of using the serum antibody levels of mice immunized with 0.5mL dose to replace the animal evaluation of foot and mouth disease vaccine eficacy is the highest.

Key WordsMouse serological antibodies;Target animals;Foot and mouth disease vaccine effcacy

口蹄疫（footandmouthdisease，FMD）是由口蹄疫病毒（footandmouthdiseasevirus，FMDV）感染引起的偶蹄动物共患的一种急性、热性传染病，主要病理特征为患病动物的口、舌、唇、蹄、乳房等部位发生水泡，破溃形成烂斑，以流行广、传播快、发病率高而著称[1]，被世界动物卫生组织（WOAH）列为必报烈性传染病，我国也将其病列为一类动物传染病之首。（剩余3989字）