甜瓜种子内生菌多样性及其促生功能分析

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Abstract：Toidentifyendophytesinmelon（CucumismeloL.）seedsand verifytheirplant growth-promoting functions， melon seeds were used as the experimental materials.Endophytes were isolated using the dilution plating method，and theirdiversitywasanalyzedbasedon16SrRNAgenesequencing.Theisolatedendophytes wereevaluatedfortheirabilitiesinphosphatesolubilation，trogenfation，potassmsolubilation，produtionofdole--aceticacid（A）derophore production，andcelulose decompositionusing various growth-promoting media.Theresultsshowed that39 bacterial strainseresccessfullysolatedfromthemelonseeds，elongingto7speciesacross3gneraBacilus，Esric ia，Shigella），ithBacillusbingthdominantgenus.AllspeciesexibiteditrogenfixationandAAproductioncapabilities，while5 species belonging totheBacillus andEscherichia genera demonstrated siderophores production.In conclusion，the melonseeds hrboreddiverseendophytes，andallendophytespossssgrowth-promoting functions.Thisstudyreveals the diversityof endophyte in melon seeds and provides valuable germplasm resources for the development of environmentally friendlybiofertilizers.

Keywords：Melon seeds;Endophytes;Diversity;Plant growth promotion

植物内生菌指在其生活史的一定阶段或所有阶段定殖于健康植物组织内部或细胞间隙，而宿主植物没有表现出明显病害症状的微生物，包括内生细菌、真菌和放线菌等。（剩余13240字）